Differential expression of microRNAs in canine triple-negative tubulopapillary carcinoma

• Grant number: 19/20071-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2020
• End date: October 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Flavia Lombardi Lopes
• Grantee: Nayra Cristina Herreira do Valle
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Canine mammary tumors (CMT) are often found in veterinary medicine routine and is, therefore, a subject that allows and requires further investigation. Advances in biotechnology have allowed us to investigate the exact role of extrinsic and intrinsic mechanisms involved in oncogenesis of this condition that affects thousands of animals, including dogs and cats, as well as humans. The dog is considered to be a model of this disease and translational research is mutually beneficial. Thus, the objective is to analyze the differential expression of microRNAs in triple-negative tubulopapillary carcinoma in bitches; and to identify their specific targets and canonical pathways. To this end, female dogs from the hospital routine, presenting spontaneous mammary tumors were selected. Samples were taken from bilateral therapeutic radical mastectomy. The fragments are being processed for total RNA extraction and microtranscriptome analysis using the microarray technique and then in silico analysis will be performed to identify targets and enrich the biological pathways, enabling the identification of diagnostic and therapeutic targets. (AU)