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Differential expression of microRNAs in canine triple-negative tubulopapillary carcinoma

Grant number: 19/20071-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2020
Effective date (End): October 31, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal researcher:Flavia Lombardi Lopes
Grantee:Nayra Cristina Herreira do Valle
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Canine mammary tumors (CMT) are often found in veterinary medicine routine and is, therefore, a subject that allows and requires further investigation. Advances in biotechnology have allowed us to investigate the exact role of extrinsic and intrinsic mechanisms involved in oncogenesis of this condition that affects thousands of animals, including dogs and cats, as well as humans. The dog is considered to be a model of this disease and translational research is mutually beneficial. Thus, the objective is to analyze the differential expression of microRNAs in triple-negative tubulopapillary carcinoma in bitches; and to identify their specific targets and canonical pathways. To this end, female dogs from the hospital routine, presenting spontaneous mammary tumors were selected. Samples were taken from bilateral therapeutic radical mastectomy. The fragments are being processed for total RNA extraction and microtranscriptome analysis using the microarray technique and then in silico analysis will be performed to identify targets and enrich the biological pathways, enabling the identification of diagnostic and therapeutic targets. (AU)