Diversity and functional roles of yeasts in fungus-growing insects

• Grant number: 19/24412-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2020
• End date: October 31, 2023
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: André Rodrigues
• Grantee: Rodolfo Bizarria Júnior
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated research grant: 19/03746-0 - Collaborative research: Dimensions US-São Paulo: integrating phylogeny, genetics, and chemical ecology to unravel the tangled bank of the multipartite fungus-farming ant symbiosis, AP.BTA.TEM
• Associated scholarship(s): 21/09980-4 - Double-stranded RNA virus associated with Saccharomycotina yeast lineages, BE.EP.DR

Abstract

Many yeasts live in association with insects, playing a role in detoxification and chemical signaling, protection against antagonists and, eventually, in larval development. Several studies revealed the large amount of yeasts in fungus-growing insects, which are considered biological models to study insect-fungus symbioses. Nevertheless, the diversity of yeast in these systems is still poorly addressed. The present project aims at describing the diversity of yeasts and dimorphic fungi in fungus-growing insects. Attine ants (Hymenoptera: Myrmicinae: Attina) and ambrosia beetles (Coleoptera: Curculionidae: Platypodinae) will be used as study models. Moreover, yeasts will be evaluated for Killer toxin production. Such toxins play a role in fungal communities, by interference competition towards sensitive strains, and can elucidate the identification of predominant microbes in this environment. Symbiotic fungi associated with beetles (Ophiostomatales, Microascales, and occasionally Hypocreales: Fusarium) will be evaluated for gene polymorphisms that encode for plant polymer-degrading enzymes. Such analyses may reveal selective pressures that tighten the symbioses between beetles and fungi. Therefore, the study will generate a descriptive profile in yeast community of fungus-growing insects. Additionally, it will reveal the occurrence of killer yeasts in such environments and the gene polymorphism associated with the beetles-fungus symbiosis. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BIZARRIA, RODOLFO; KOOIJ, PEPIJN W.; RODRIGUES, ANDRE. Climate Change Influences Basidiome Emergence of Leaf-Cutting Ant Cultivars. JOURNAL OF FUNGI, v. 7, n. 11, NOV 2021. (19/24412-2, 19/03746-0)

BIZARRIA JR, RODOLFO; PIETROBON, TATIANE DE CASTRO; RODRIGUES, ANDRE. Uncovering the Yeast Communities in Fungus-Growing Ant Colonies. MICROBIAL ECOLOGY, v. N/A, p. 12-pg., 2022-08-12. (19/03746-0, 19/24412-2)

ZUVANOV, LUIZA; BASSO GARCIA, ANA LETYCIA; CORRER, FERNANDO HENRIQUE; BIZARRIA JR, RODOLFO; DA COSTA FILHO, AILTON PEREIRA; DA COSTA, ALISSON HAYASI; THOMAZ, ANDREA T.; MENDES PINHEIRO, ANA LUCIA; RIANO-PACHON, DIEGO MAURICIO; WINCK, FLAVIA VISCHI; et al. he experience of teaching introductory programming skills to bioscientists in Brazi. PLOS COMPUTATIONAL BIOLOGY, v. 17, n. 11, NOV 2021. (17/21983-3, 19/10727-1, 15/03541-8, 20/02084-0, 19/24412-2, 17/10373-0, 19/07526-4, 20/04437-8)

BIZARRIA, JR., RODOLFO; PAGNOCCA, FERNANDO CARLOS; RODRIGUES, ANDRE. easts in the attine ant-fungus mutualism: Diversity, functional roles, and putative biotechnological application. YEAST, v. 39, n. 1-2, SI, SEP 2021. (19/24412-2, 19/03746-0)