Unrevealing the role of plant growth-promoting bacteria in recruiting microbiome from maize rizosphere

• Grant number: 19/25720-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2020
• End date: September 10, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Maria Carolina Quecine Verdi
• Grantee: Jessica Aparecida Ferrarezi
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

The beneficial plant-microbes association have been widely explored with the purpose of understanding the mechanisms involved in this interaction, in order to develop bioinoculants for agriculture aiming at sustainability and reduction of chemical inputs use. Plant Growth Promoting Bacteria (PGPBs) have been reported as interesting alternatives to mineral fertilizers for a range of crops. The strain Bacillus sp. RZ2MS9 and the genus Azospirillum have shown potential in plant growth promotion, and for that they are called PGPBs. However, the lack of holistic comprehension about PGPBs-plant-native soil microbiota can lead to inconsistency of results in field conditions. Recent ecological theories reveal that plant microbiomes are organized as microbial hubs with keystone species and helpers. These microbial networks are strongly connected, molecularly modulated and can influence plant metabolism and physiology. The molecular modulation of such association can be accessed with the advance of high throughput sequencing and phenotyping technologies. It has been possible to access information about the plant-associated bacterial community (including those non-cultivable), correlate it with the main microbiota-modulating factors, such as plant genotype, growth stage and physiological responses under influence of biostimulants. Thus, through metagenomics, metatranscriptomics, metaproteomics and phenomics, the objective of this project is to characterize the microbial community associated with 3 maize genotypes under influence of the potential keystone PGPBs Bacillus sp. RZ2MS9 and A. brasilense Ab-v5 to support the original development of a synthetic minimum microbiome including these two species as keystones for plant growth promotion, aiming at the construction of a bacterial consortium. This unprecedent omics approach for the holistic view of the complex system composed by inoculant-plant-microbiota and data integration will allow the development of new strategies for optimization of plant biostimulats recommendation with enhanced efficiency for establishment in the field. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRAREZI, JESSICA A.; MCTAGGART, ALISTAIR R.; TOBIAS, PERI A.; HAYASHIBARA, CAROLINA A. A.; DEGNAN, REBECCA M.; SHUEY, LOUISE S.; FRANCESCHINI, LIVIA M.; LOPES, MARIANA S.; QUECINE, MARIA C.. Austropuccinia psidii uses tetrapolar mating and produces meiotic spores in older infections on Eucalyptus grandis. Fungal Genetics and Biology, v. 160, p. 8-pg., 2022-05-01. (14/16804-4, 19/25720-2)

FERRAREZI, JESSICA APARECIDA; CARVALHO-ESTRADA, PAULA DE ALMEIDA; BATISTA, BRUNA DURANTE; ANICETO, RAFAEL MARTINS; PROHMANN TSCHOEKE, BRUNO AUGUSTO; DE MAIA ANDRADE, PEDRO AVELINO; LOPES, BRUNA DE MOURA; BONATELLI, MARIA LETICIA; ODISI, ESTACIO JUSSIE; AZEVEDO, JOAO LUCIO; et al. Effects of inoculation with plant growth-promoting rhizobacteria from the Brazilian Amazon on the bacterial community associated with maize in field. APPLIED SOIL ECOLOGY, v. 170, FEB 2022. (15/01188-9, 19/04697-2, 19/25720-2)

FERRAREZI, JESSICA APARECIDA; DEFANT, HELOISA; DE SOUZA, LEANDRO FONSECA; AZEVEDO, JOAO LUCIO; HUNGRIA, MARIANGELA; QUECINE, MARIA CAROLINA. Meta-omics integration approach reveals the effect of soil native microbiome diversity in the performance of inoculant Azospirillum brasilense. FRONTIERS IN PLANT SCIENCE, v. 14, p. 15-pg., 2023-06-29. (19/25720-2, 19/04697-2, 21/11983-1)

HAYASHIBARA, CAROLINA ALESSANDRA DE ALMEIDA; LOPES, MARIANA DA SILVA; TOBIAS, PERI A.; SANTOS, ISANELI BATISTA DOS; FIGUEREDO, EVERTHON FERNANDES; FERRAREZI, JESSICA APARECIDA; MARQUES, JOAO PAULO RODRIGUES; MARCON, JOELMA; PARK, ROBERT F.; TEIXEIRA, PAULO JOSE PEREIRA LIMA; et al. In Planta Study Localizes an Effector Candidate from Austropuccinia psidii Strain MF-1 to the Nucleus and Demonstrates In Vitro Cuticular Wax-Dependent Differential Expression. JOURNAL OF FUNGI, v. 9, n. 8, p. 16-pg., 2023-08-01. (14/16804-4, 19/25720-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERRAREZI, Jessica Aparecida. Unraveling the role of plant growth-promoting bacteria in recruiting microbiome from maize rhizosphere. 2024. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.