Cellular effects of chaperone inhibition by violacein

• Grant number: 20/01890-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2020
• End date: April 25, 2023
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Elizabeth Bilsland
• Grantee: Beatriz Yumi Hata
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/18611-7 - Development of new tools for search and validation of molecular targets for therapy against Plasmodium vivax, AP.TEM

Abstract

Violacein is a natural compound with antibacterial, antiparasitic and antitumoral activities. Several published studies show evidence that violacein induces apoptosis, however the mechanism involved in it is still unknown. Using chemical genomic profiling in yeast, we identified direct targets of violacein: Hsp70 chaperones. The present work aims to elucidate cellular effects of Hsp70 chaperone inhibition on yeast and mammalian cells.