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Predicted state of H3K27me3 histone modification in Nellore cattle muscle as an indicator of gene modulation associated with mineral amount and meat quality traits

Grant number: 19/25639-0
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): January 01, 2021
Effective date (End): December 31, 2023
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal Investigator:Luciana Correia de Almeida Regitano
Grantee:Juliana Afonso
Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
Associated research grant:19/04089-2 - The Nelore hologenome: implications in beef quality and feed efficiency, AP.ESCIENCE.TEM
Associated scholarship(s):22/04393-6 - Investigation on the H3K27me3 histone modification-based gene expression repression associated to methane emission in Nelore cattle rumen tissue, BE.EP.PD


Several factors affect beef quality, with tenderness, the amount of intramuscular fat and the nutritional values some of the attributes used as a criterion of choice by consumers. Despite the previous identification of genes and genomic regions involved with the prementioned traits, the mechanisms of gene regulation affecting these genes are not yet fully known. Therefore, our objective is to predict in silico genes that are discordantly activated, based on the predicted absence of the H3K27me3 hysteric modification, whose presence causes gene silencing. This analysis will be carried out between Longissimus thoracis muscle samples of Nelore steers contrasting for mineral amount and beef quality traits, in order to relate the observed patterns to the phenotypes of interest to breeding. For this, a repressive tendency score related to the predicted absence of H3K27me3 will be calculated for each of the genes expressed, homologous to human genes, in each of the of 113 Nelore samples, using a discordant score, a method developed for human genes by an Australian research group, collaborators of our project. Based on this score, we are proposing a new methodology in which the genes of each sample will be ranked and the ranking between contrasting sample groups regarding the studied phenotypes will be compared to determine which genes are more discordantly activated between them. Analyses with the Partial Correlation with Information Theory (PCIT) and Regulatory Impact Factor (RIF) algorithms will be performed to obtain the genes correlated to tenderness and intramuscular fat and their potential regulatory impacts on these characteristics, complementing analysis already implemented for mineral amount. Functional analyses will be carried out in all lists of genes discordantly activated. The results will be integrated with data already obtained from transcription factors expressed in our samples, genes being affected by cis and trans eQTLs and differentially expressed or with expression values correlated to the phenotypes in order to achieve a wider range of regulatory events affecting each gene. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AFONSO, JULIANA; SHIM, WOO JUN; BODEN, MIKAEL; FORTES, MARINA RUFINO SALINAS; DINIZ, WELLISON JARLES DA SILVA; DE LIMA, ANDRESSA OLIVEIRA; ROCHA, MARINA IBELLI PEREIRA; CARDOSO, TAINA FIGUEIREDO; BRUSCADIN, JENNIFER JESSICA; GROMBONI, CAIO FERNANDO; et al. Repressive epigenetic mechanisms, such as the H3K27me3 histone modification, were predicted to affect muscle gene expression and its mineral content in Nelore cattle. BIOCHEMISTRY AND BIOPHYSICS REPORTS, v. 33, p. 9-pg., . (19/25639-0, 19/04089-2)

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