Structural analysis and relative expression of the HCMV IL0 isoforms in glioblastoma samples

• Grant number: 20/07767-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2020
• End date: November 30, 2021
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Maria Cristina Carlan da Silva
• Grantee: Tainan Cerqueira Neves
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Human Cytomegalovirus (HCMV) belongs to the Herpesviridae family,Betaherpesvirinae subfamily, which includes neurotropic viruses, with long replicative cycle and restricted species specificity. HCMV is an agent with high worldwide prevalence endowed with an exceptionally large arsenal of coded proteins which evade the host's innate and adaptive immune defenses, promoting viral persistence and latency throughout the host's life. Latency is characterized by the presence of viral DNA specific cell types with absence of viral replication and particle formation. A group of immunomodulatory proteins expressed by HCMV are homologous of host cytokines and chemokines. The cellular interleukin-10 homologue (cIL-10), called viral IL-10 (cmvIL-10). The coding gene of cmvIL-10, UL111A, undergoes alternative splicing producing different isoforms. The transcript cmvIL10 (Isoform A) is expressed mainly during the lytic cycle, while LAcmvIL10 (Isoform B) is expressed in the lytic cycle and during latency. In addition, to these two transcripts there are another 5 transcripts (C - G) reported. However, little information about its functions and structures are known. The present project aims, the computational modeling and interaction analysis between the isoforms A and B with the il10 cell receptor. In addition to the comparation of IL-10 expression of isoforms A, B, E and F.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PANTALEAO, SIMONE QUEIROZ; BOMEDIANO CAMILLO, LIVIA DE MORAES; NEVES, TAINAN CERQUEIRA; MENEZES, ISABELA DE GODOY; STANGHERLIN, LUCAS MATHEUS; DE CARVALHO RUTHNER BATISTA, HELENA BEATRIZ; POOLE, EMMA; NEVELS, MICHAEL; PHILOT, ERIC ALISSON; SCOTT, ANA LIGIA; et al. Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor. PLoS One, v. 17, n. 11, p. 17-pg., 2022-11-28. (20/07767-9, 20/08527-1)