Searching for new molecular tools to enhance Pseudomonas putida robustness to abiotic stresses

• Grant number: 19/25432-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2020
• End date: February 28, 2025
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: María Eugenia Guazzaroni
• Grantee: Guilherme Marcelino Viana de Siqueira
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries, AP.JP
• Associated scholarship(s): 22/12050-1 - Genome-wide identification of membrane transporters for biofuel tolerance in Pseudomonas putida using adaptive laboratory evolution, BE.EP.DR

Abstract

Research and development of new biocatalysts are imperative for the establishment of sustainable industrial processes; however, the limited toolbox for reliable genetic manipulation of non-conventional microbial hosts is still a limiting factor for the industrial employment of microorganisms with great biotechnological potential. Recent advances in sequencing and data analysis technologies have become a valuable source of biological information, and, thus, a wide range of new genes and biological functions from uncultivable bacteria are provided for synthetic biologists and metabolic engineers to explore. This project aims to utilize biological data mining to unearth new tools for genetic manipulation of Pseudomonas putida, a Gram-negative bacteria with emerging industrial properties, as well as new genes able to confer greater robustness for microorganisms grown under the extreme conditions needed in industrial fermentation processes, allowing the development of novel biocatalysts suitable for ventures aiming the expansion of life's boundaries.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SIQUEIRA, GUILHERME MARCELINO VIANA; GUAZZARONI, MARIA-EUGENIA. Host-Dependent Improvement of GFP Expression in Pseudomonas putida KT2440 Using Terminators of Metagenomic Origin. ACS SYNTHETIC BIOLOGY, v. 12, n. 5, p. 5-pg., 2023-05-01. (19/25432-7, 21/01748-5)

KIKUTI MANCILIO, LUCCA BONJY; RIBEIRO, GUILHERME AUGUSTO; RODRIGUES DE ALMEIDA, ERICA JANAINA; VIANA DE SIQUEIRA, GUILHERME MARCELINO; ROCHA, RAFAEL SILVA; GUAZZARONI, MARIA-EUGENIA; DE ANDRADE, ADALGISA RODRIGUES; REGINATTO, VALERIA. Adding value to lignocellulosic byproducts by using acetate and p-coumaric acid as substrate in a microbial fuel cell. INDUSTRIAL CROPS AND PRODUCTS, v. 171, NOV 1 2021. (18/05454-3, 19/25432-7, 18/15528-4, 14/50945-4, 18/12471-1, 19/15675-0, 15/04309-1)