Analysis of the levels of inflammatory and anti-inflammatory ocitocina, in orthodontic mini-implants, submitted to orthodontic load

• Grant number: 20/10095-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2021
• End date: June 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Maria Bernadete Sasso Stuani
• Grantee: Mariah Carboni Mendes
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Little is known about the inflammatory response to miniscrew loading. Better understanding of the biomarkers involved in this process may help to reduce the risk factors that can lead to the premature loss of the miniscrew. Objectives: The aim of this study will be to evaluate the levels of IL-1±, IL-1², IL-1Ra, IL-10, IL-13 in the peri-miniscrew implant crevicular fluid (MICF). Methods:The study sample will be comprise 20 immediately loaded (Group I) and 20 unloaded miniscrews (Group II). A load in the range of 150 to 200gF will be immediately applied to the miniscrews in the loaded group after the placement surgery. MICF was collected at three different time points: at baseline (T0), at day 7 (T2) and at day 14 (T3). The levels of the biomarkers will be measured using a multiplexed bead immunoassay. Intergroup comparisons will be made using Mann-Whitney test. Friedman and Dunn's multiple comparison tests will be used to evaluate intragroup differences over time.