In the health area, this project has a strong component of newbiotechnologies and advanced solutions for the production of productsbiopharmaceuticals to be used as therapeutic agents in various typesof cancer, specifically, in leukemia. L-asparaginase (E.C.220.127.116.11)(ASNase) is the enzyme that catalyzes the hydrolysis reaction of the amino group ofside chain of the amino acid L-asparagine, releasing aspartic acid and ammonia,being widely used as an anti-neoplastic agent. This enzyme can beproduced by a series of microorganisms, however, there is a need fornew producing sources with higher yields and less adverse effects.Thus, the objective of this project is to evaluate the production of L-asparaginase by astrain of Bacillus subtilis that produces Aliivibrio fischeri L-Asparaginase IIrotary shaker and scale up the process for tank bioreactoragitated. Initially, a study will be carried out on a rotary shaker evaluating,induction time, inductor concentration, inoculum size andcultivation. In parallel, cell disruption tests will be carried out toimprove extraction of the intracellular enzyme. Subsequently, scale up will be doneprocess for agitated tank bioreactor and some parameters will be evaluatedhydrodynamics related to the production process. The development ofnew L-asparaginase production lines and production process,will allow to obtain a therapy with higher yields, more effective and withreduced side effects.
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