Genetic characterization of isolated microorganisms in the surgical site of dogs and in the surgical environment

• Grant number: 19/20585-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2021
• End date: February 28, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Paola Castro Moraes
• Grantee: Mareliza Possa de Menezes
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 21/09438-5 - LAMP point-of-care testing to guide antimicrobial choice for treatment of canine pyoderma, BE.EP.DR

Abstract

The bacterin resistance event is a natural phenomenon and its development is inevitable at some point, but the undue use of antimicrobials has contributed to its evolution due to the great capacity of adaptation of these microorganisms in a short period of time. Infections caused by multidrug resistant organisms are associated with increased morbidity, mortality, and a significant increase in health care expenditures. Within this scenario, Veterinary Medicine plays an important role in the development of resistant microorganisms and presents great potential for transfer of genes and microorganism from veterinary patients to humans, and the use of antimicrobials in prophylaxis and surgical therapy is one of the main reasons for the use of these drugs in company animals. In this sense, the present project proposes the evaluation of the microbiological profile of the surgical site, surgeon's hand before and after hand's antisepsis and surgery environment during the transoperative period of clean, clean -contaminated and contaminated surgeries, through the sequencing of the 16S rRNA and the profile in pulsed-field gel electrophoresis of the microorganisms isolated in the surgical site and surgical environment, what can contribute to associate or not the infection of surgical site to the bacterial population present in the environment and surgical team, allowing the identification of undesired growth, potential of transmission of microorganisms between patients and veterinary professionals, anticipation of the knowledge of the bacterial population in a certain procedure and identification of multiresistant organisms present in the hospital environment, which may help in the establishment of protocols for veterinary hospital environment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENEZES, MARELIZA P.; BORZI, MARIANA M.; RUARO, MAYARA A.; CARDOZO, MARITA, V; RABELO, RODRIGO C.; VERBISCK, NEWTON V.; MORAES, PAOLA C.. Multidrug-Resistant Bacteria Isolated From Surgical Site of Dogs, Surgeon's Hands and Operating Room in a Veterinary Teaching Hospital in Brazil. TOPICS IN COMPANION ANIMAL MEDICINE, v. 49, p. 6-pg., 2022-07-01. (19/20585-0)