Role of the Migratory Bird Dendrocygna viduata on the Dissemination of Antimicrobial-Resistant Gram-negative Rods

• Grant number: 17/02258-6
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2017 - October 31, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ana Cristina Gales
• Grantee: Ana Cristina Gales
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Joao Batista da Cruz ; Patrícia Locosque Ramos

Abstract

The use and misuse of antimicrobial agents in the human medicine, agriculture and animal husbandry have contributed for the emergence of resistant bacteria. Resistant bacteria have been more commonly detected in the hospital setting; however, multi-drug resistant isolates are also currently found in the community. Several studies have demonstrated that migratory birds play an important role in the ecology, circulation and dissemination of antimicrobial resistance encoding genes, acting as efficient vectors of these resistance determinants. In Brazil, a few studies have evaluated the role of migratory birds in the dissemination of infectious diseases agents but, to our knowledge, none of them evaluated the presence of antimicrobial resistance encoding genes. Some species of Brazilian migratory birds migrate to São Paulo state, including the surrounding areas of the São Paulo Zoo Foundation Lake. It is estimated that 3,000 migratory birds visit the Zoo looking for food and shelter during the winter season. Among them, Dendrocygna viduata represents one of the most frequently species encountered in this area. A total of 1,000 strict and facultative aerobic bacterial isolates were recovered from cloacal and choanal swabs collected from D. viduata that visited the lake of São Paulo Zoo area between July and August 2012. The swabs were inoculated onto CHROMagarTM Orientation Medium. Each colony showing distinct color in CHROMagarTM plates was stored in cryotubes containing TSB supplemented with 15% glycerol at -20ºC in the ALERTA Laboratory bacterial collection for further characterization. The main objectives of this study will be: i) the identification of all GNB showing resistance to ²-lactams, quinolones, aminoglycosides and polymyxin B at the species level by MALDI-TOF and/or 16S rRNA sequencing; ii) the determination of the antimicrobial susceptibility profile (ASP) of Gram-negative bacilli (GNB) isolated from cloacal and choanal swabs; iii) the detection and characterization of genes encoding resistance to ²-lactams, quinolones, aminoglycosides and polymyxin B by PCR followed by DNA sequencing; iv) evaluation of genetic relatedness by PFGE among resistant GNB isolates; vi) characterization of the location and the genetic context of antibiotic resistance genes. Up to date, resistance to different antimicrobials was evaluated in 329 isolates by disk-diffusion. Resistance to cefotaxime, imipenem, gentamicin, ciprofloxacin and polimyxyn B was observed in 22,8% (n=75), 5,2% (n=17), 16,7% (n=55), 14,8% (n=49) and 11,5% (n=38) of the isolates, respectively. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, WILLAMES M. B. S.; NARCISO, ANA CLARA; CAYO, RODRIGO; SANTOS, STEFANIE VANESSA; FEHLBERG, LORENA C. C.; RAMOS, PATRICIA LOCOSQUE; DA CRUZ, JOAO BATISTA; GALES, ANA CRISTINA. SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 90, n. 3, p. 221-227, MAR 2018. (14/12224-3, 17/02258-6)

LENZI, MICHAEL H.; MARTINS, WILLAMES M. B. S.; ROCH, MELANIE; RAMOS, PATRICIA L.; SANDS, KIRSTY; CAYO, RODRIGO; WALSH, TIMOTHY R.; ANDREY, DIEGO O.; GALES, ANA C.. A new mutation in mgrb mediating polymyxin resistance in Klebsiella variicola. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, v. 58, n. 5, NOV 2021. (17/02258-6, 18/24431-4)

NARCISO, ANA CLARA; MARTINS, WILLAMES M. B. S.; ALMEIDA, LUIZ G. P.; CAYO, RODRIGO; SANTOS, STEFANIE VANESSA; RAMOS, PATRICIA LOCOSQUE; LINCOPAN, NILTON; VASCONCELOS, ANA TEREZA R.; GALES, ANA CRISTINA. Healthcare-associated carbapenem-resistant OXA-72-producing Acinetobacter baumannii of the clonal complex CC79 colonizing migratory and captive aquatic birds in a Brazilian Zoo. Science of The Total Environment, v. 726, JUL 15 2020. (17/02258-6)

NARCISO, ANA CLARA; MARTINS, WILLAMES M. B. S.; CAYO, RODRIGO; DE MATOS, ADRIANA PEREIRA; SANTOS, STEFANIE VANESSA; RAMOS, PATRICIA LOCOSQUE; DA CRUZ, JOAO BATISTA; GALES, ANA CRISTINA. Detection of OXA-58-Producing Acinetobacter seifertii Recovered from a Black-Necked Swan at a Zoo Lake. Antimicrobial Agents and Chemotherapy, v. 61, n. 12, DEC 2017. (17/02258-6)