Cowpea mild mottle virus in soybean: virus-vector interaction, incidence and associated vector, virus variability and impacts on physiological quality of seeds from diseased plants

• Grant number: 19/24642-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2021
• End date: October 31, 2022
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Renate Krause Sakate
• Grantee: Felipe Barreto da Silva
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Soybean (Glycine max L.) is one of the main agricultural commodities in Brazil as a source of vegetal oil and protein. In the last years, soybean production has been highly attacked by the whitefly Bemisia tabaci, that is a pest and vector of important viruses such as the Carlavirus Cowpea Mild Mottle Virus (CPMMV), causal agent of the soybean stem necrosis, diseased reported in Brazil in the 2000/2001 season. Little is known about the losses caused by CPMMV in the current cultivars planted in Brazil, as well as the aspects related to the virus and vector interaction. Therefore, this project aims to evaluate the effects of CPMMV in the soybean yield considering the infection in different phenological stages of the plant; to evaluate the sanity and physiology of seed from diseased plants; the virus variability in different regions of Brazil; the transmission of CPMMV in the main genotypes currently planted, and aspects related between the interaction of CPMMV and B. tabaci. Viral variability will be analysed by High Throughput Sequencing (HTS) to obtain the complete genome of different CPMMV isolates and further phylogenetic analyses. HTS data will also verify the occurrence of other viruses associated to the soybe an crop. The results obtained in this study will enable a better understanding of the damages caused by CPMMV in soybean and the disease epidemiology in the field. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO-JUNIOR, MARCOS ROBERTO; DA SILVA, FELIPE BARRETO; MARUBAYASHI, JULIO MASSAHARU; UZAN, JULIANA; NOGUEIRA, ANGELICA MARIA; MULLER, CRISTIANE; NASCIMENTO, DANIELE MARIA; YUKI, VALDIR ATSUSHI; NARITA, NOBUYOSHI; PAVAN, MARCELO AGENOR; et al. Molecular and biological characterization of an isolate of the potyvirus passiflora virus Y naturally infecting soybean (Glycine max) in Brazil. ARCHIVES OF VIROLOGY, v. N/A, p. 5-pg., 2022-09-21. (19/18639-4, 19/24642-8)