Use of commercial vacinnes for porcine circovirus 2 in a conventional farm: impact on PCV3 viral load in feces and serum

• Grant number: 20/15758-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2021
• End date: July 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Alessandra Marnie Martins Gomes de Castro
• Grantee: Rosecleer Rodrigues da Silva
• Host Institution: Faculdade Alvorada Saúde. São Paulo , SP, Brazil

Abstract

The circoviruses that infect pigs belongs to the family Circoviridae, genus Circovirus. The viruses are icosahedral morphology viruses of about 17 nm in diameter, non-enveloped, with a genome consisting of circular single-stranded DNA (ssDNA). The genus Circovirus is composed of 29 viral species that infect animals, including the three that infect pigs: Porcine circovirus 1 (PCV1); Porcine circovirus 2 (PCV2); Porcine circovirus 3 (PCV3); and Porcine circovirus 4 (PCV4), recently discovered; these three species that infect pigs has been identified in several countries of the world. PCV1 is non-pathogenic for pigs. PCV2 is the main etiological agent of PMWS in pigs that causes anemia, diarrhea, jaundice and enlarged lymph nodes, resulting in weight loss and PCV2 has been associated with of a great economic loss in the pig industry. PCV3, the main agent of this study, induces multisystemic heart disease and inflammation in pigs, has been reported for the first time in the United States and more recently in Brazil. Considering that Brazil is currently one of the largest pork exporters and that there is little information on the impact of vaccination for PCV3, the present study aims to: (I) quantify PCV3 DNA; (II) standardize a PCV3 detection system by quantitative PCR (PCRq); (III) test the collected samples. Serum and feces samples from 76 animals collected on days 0, 14, 28, 56 and 70 will be analyzed after vaccination for PCV2, totaling 760 samples. This study will provide information on the impact of vaccination on the persistence and / or increase of PCV3 viral circulation and, the results obtained will allow to determine the effect of PCV2 vaccination on viremia and PCV3 elimination, thus contributing to the knowledge of the impact of the vaccine in the viral circulation.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

V.H. SILVA; D.F. SILVA; F. BALDISSERI; J.C.S. BORGES; L.J. BORBA; R.R. SILVA; A.M.M.G. CASTRO. PCV2: The unexpected threat - How fish infection can transform industries and society: A pilot study. SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE, v. 54, n. 1, p. 93-99, 2024-00-00. (17/21592-4, 20/15758-0)