Modulating photochemical reactions in Escherichia coli lipid monolayers with the binding mechanisms of eosin decyl ester and toluidine blue-O photosensitizers

• Grant number: 20/15854-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): April 01, 2021
• Effective date (End): August 31, 2023
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Pedro Henrique Benites Aoki
• Grantee: Lucas Gontijo Moreira
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 22/01971-9 - Revealing the impact of dimethyl methylene blue cellular localization to the photodynamic efficiency in tumoral cells derived from skin, BE.EP.MS

Abstract

Photodynamic damage to the cell envelope can inactivate microorganisms and may be used as a tool to combat the phenomenon of super-resistance, empowered by the indiscriminate use of antibiotics. Efficiency in microbial inactivation is dependent on the incorporation of photosensitizers (PS) into the bacterial membranes to trigger oxidation reactions under illumination. Therefore, we shall build Langmuir monolayers of Escherichia coli lipid extract to determine the binding mechanisms and oxidation outcomes induced by eosin decyl ester (EosDEC) and toluidine blue-O (TBO). Incorporation of the PS will be accessed by surface-pressure isotherms of the E. coli monolayers while Fourier-transform infrared spectroscopy (FTIR) of Langmuir-Schaefer (LS) films will provide information on the PSs binding mechanisms. Photochemical reactions will be triggered by light irradiation of the Langmuir monolayers at 30 mN/m, evaluated by means of the surface area evolution. The goal is to address the role played by the molecular interactions and PS binding site to the photochemical outcome, which is relevant for the efficiency in photodynamic inactivation of microorganism (PDIM) applications. This research project is inserted in the Thematic Project n° 2018/22214-6. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA JR, ALEXANDRE M.; MOREIRA, LUCAS G.; CAMACHO, SABRINA A.; FERREIRA, FABIANA G.; CONCEICA, KATIA; TADA, DAYANE B.; AOKI, PEDRO H. B.. Photochemical outcomes triggered by gold shell-isolated nanorods on bioinspired nanoarchitectonics for bacterial membranes. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1865, n. 8, p. 10-pg., 2023-08-25. (18/14692-5, 20/15854-9, 18/22214-6)

CAMACHO, SABRINA A.; KOBAL, MIRELLA B.; MOREIRA, LUCAS G.; BISTAFFA, MARIA J.; ROQUE, THAMIRES C.; PAZIN, WALLANCE M.; TOLEDO, KARINA A.; OLIVEIRA JR, OSVALDO N.; AOKI, PEDRO H. B.. The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 211, MAR 2022. (18/14692-5, 18/16713-0, 20/12129-1, 20/15854-9, 14/11408-3, 17/03879-4, 18/22214-6)

MOREIRA, LUCAS G.; ALMEIDA JR, ALEXANDRE M.; NIELD, TYLER; CAMACHO, SABRINA A.; AOKI, PEDRO H. B.. Modulating photochemical reactions in Langmuir monolayers of Escherichia coli lipid extract with the binding mechanisms of eosin decyl ester and toluidine blue-O photosensitizers. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 218, MAY 2021. (20/15854-9, 18/16713-0, 18/22214-6, 18/14692-5)

KOBAL, M. B.; CAMACHO, S. A.; MOREIRA, L. G.; TOLEDO, K. A.; TADA, D. B.; AOKI, P. H. B.. Unveiling the mechanisms underlying photothermal efficiency of gold shell-isolated nanoparticles (AuSHINs) on ductal mammary carcinoma cells (BT-474). Biophysical Chemistry, v. 300, p. 9-pg., 2023-09-01. (18/14692-5, 18/16713-0, 21/14500-1, 20/15854-9, 14/11408-3, 18/22214-6, 22/02189-2, 17/03879-4)