Comparison of the in vitro response between treatments with resveratrol viscum album either-or doxorubicin in canine osteosarcoma cell culture

• Grant number: 20/01639-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2021
• End date: July 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Renee Laufer Amorim
• Grantee: Felipe César da Silva Brasileiro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Osteosarcoma is a locally aggressive bone neoplasm, highly metastatic, and with an unfavorable prognosis. It represents 80% of canine malignant bone tumors, more frequent in large males, and with an average age of seven and a half years. By sharing multiple biological aspects with human osteosarcoma, the dog is a good model of comparative oncopathology for genetic, epigenetic, and therapeutic studies. Since current therapeutic protocols only delay tumor progression and do not rule out an unfavorable prognosis, new alternatives should be investigated, especially those that may have lesser side effects. In this context, resveratrol and Viscum album, natural compounds that exhibited important cytotoxic and pro-apoptotic activity in human osteosarcoma (in vitro and in vivo), have been researched as therapeutic options. This study aims to verify the in vitro antitumor potential of these biopharmaceuticals in canine osteosarcoma cell culture, as well as their interaction with doxorubicin. In this study, canine appendicular osteosarcoma cells will be used in a two-dimensional primary culture already standardized, with establishment and characterization in force in a partner project (nº 2019/24468-8). There will be eight groups divided between control and treatments with resveratrol, Viscum album, and doxorubicin, alone and in combination. Analysis of cell viability and determination of IC50, migration capacity, induction of apoptosis, as well as the ability to select tumor stem cells and verify the expression of NANOG, OCT3/4, and Nestin by flow cytometry after treatment will be performed. Statistical analysis will be performed using the GraphPad Prism 5.0 program, based on parametric and non-parametric tests. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BRASILEIRO, Felipe César da Silva. Comparison of in vitro response between treatments with resveratrol, Viscum album and or doxorubicin in canine osteosarcoma cell culture. 2022. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.