Comparison of in vitro response between treatments with resveratrol, Viscum album and or doxorubicin in canine osteosarcoma cell culture

Author(s)

Felipe César da Silva Brasileiro

• Document type: Master's Dissertation
• Press: Botucatu. 2022-09-05.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2022-07-01
• Advisor: Renée Laufer Amorim; Carlos Eduardo Fonseca Alves; Flávia Karina Delella

Abstract

Osteosarcoma (OS) is a locally aggressive and metastatic neoplasm whose current therapeutic options remain ineffective in high-grade/metastatic neoplasms, both in humans and animals. Viscum album and resveratrol, natural antitumor agents, showed dose-dependent cytotoxicity and reduced the proliferation and motility of human OS cells (in vitro); but they have not been yet tested in canine and murine OS cells. This study aimed to describe the antineoplastic action of homeopathic V. album and resveratrol compared to doxorubicin. For this, canine (D17, OSA 8-4) and murine (UMR 106) osteosarcoma cell lines were cultured in vitro, subdivided into treated and control groups, followed by metabolic assessment by the cell viability technique (MTT – 24 to 72h), and of motility by the migration test (transwell - 24h). All drugs exhibited selective (dose-dependent) inhibition of cell viability in the three cell lines tested. For D17, OSA 8-4, and UMR 106 cells, the homeopathic V. album obtained IC50 of 22.38µL/mL, 25.19µL/mL, and 28.82µL/mL; resveratrol 18.52µM, 50.29µM and 24.83µM and doxorubicin 398.8nM, 777.3nM and 918.1nM, respectively. The doses found in the IC50 of the bioactive natural compounds were inhibitory cell migration and inducing apoptosis in the tested cell lines, especially resveratrol. Our results demonstrate a capacity to decrease cell viability, besides lower cell migration, a significant factor considering the great metastatic potential of OS. These results corroborate the need to work on co-test of these substances to verify the effects of synergism, antagonism, and addiction. (AU)

• FAPESP's process: 20/01639-9 - Comparison of the in vitro response between treatments with resveratrol viscum album either-or doxorubicin in canine osteosarcoma cell culture
• Grantee: Felipe César da Silva Brasileiro
• Support Opportunities: Scholarships in Brazil - Master