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Analysis of organic matter decomposing bacteria in an environment under soil acidity correction with nitrogen

Grant number: 21/01927-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2021
Effective date (End): April 30, 2022
Field of knowledge:Agronomical Sciences - Agronomy - Soil Science
Principal Investigator:Ciro Antonio Rosolem
Grantee:Luan Wesley Aparecido Pereira Alves
Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The organic matter putrefactive bacteria act on the cycling of nutrients providing the plants and the soil with a better supply of elements, such as C, P, S, and N. The presence of plant material directly influences the increase of microbial biomass, which is useful as a biological indicator of soil quality. It serves as an accelerator of answers to questions related to the changes that may occur in the soil. The relationship between soil and microorganisms may be beneficial considering that humic substances formed in the decomposition process of organic matter influence the soil aggregation aspect. This work aims to quantify and qualify the soil microbial population in a production system under application of lime and plaster, associated or not to nitrogen fertilization with soybeans and second-crop corn. The experiment will be deployed under field conditions in the city of Botucatu, state of SP, as the continuation of a long-term trial that aims to investigate the response of soybeans and second-crop corn to lime and plaster. It will also inquire about the reaction of corn to nitrogen. The treatments consist of the combination of control (without application of limestone and plaster), application of limestone, and application of limestone + plaster, in addition to four doses of N (0, 80, 160, 240 kg ha-1 of N). The soil will be sampled at depths of 0-10 and 10-20 cm. The samples will be stored in plastic bags and placed in iceboxes and subsequently frozen in a freezer at -18ºC. The Microorganisms will be identified utilizing the 16s Metagenomic Sequencing Library Preparation sequencing protocol, the sequencing will be through the Illumina MiSeq platform. The results will go through variance analysis, Tukey's test, correlation, and polynomial regression.

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