Evaluation of Adalimumab plasma levels and detection of anti-Adalimumab antibodies in patients with Crohn's Disease

• Grant number: 20/01924-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2021
• Effective date (End): August 06, 2024
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Raquel Franco Leal
• Grantee: Livia Moreira Genaro
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Crohn's Disease (CD) is a chronic inflammatory bowel disease that can result in progressive intestinal damage and disability, affecting mainly the ileal and ileocecal regions. CD can affect individuals of all ages, directly impacting the quality of life. Due to the absence of curative therapy, the current therapeutic objective is to obtain endoscopic and clinical remission, avoiding complications and future surgeries. TNF-alfa has been described as the main maintainer of the intestinal inflammatory state. The therapeutic use of Monoclonal Antibodies (MAbs), such as Adalimumab, proved to be a potent anti-inflammatory agent with fast action by binding to the TNF-alfa, neutralizing its function. Patients using MAbs may experience induction of Anti-Drug Antibodies (ADA), leading to treatment failure and exacerbation of the disease. Thus, therapeutic optimization, based on the serum dosage of MAbs and measurement of the levels of ADA antibodies, has proved to be relevant. The present study aims to quantify the serum levels of Adalimumab and the levels of anti-Adalimumab antibodies in patients with CD of the Clinical Hospital from the University of Campinas (UNICAMP). These analyses may elucidate one of the possible mechanisms acting on therapeutic failure, in addition to being used as a basis for therapeutic drug monitoring to maximize endoscopic response and to minimize side effects. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GENARO, LIVIA MOREIRA; MIANI GOMES, LUIS EDUARDO; MENEZES DE FREITAS FRANCESCHINI, ANA PAULA; CECCATO, HUGO DUGOLIN; DE JESUS, RAFAEL NASCIMENTO; LIMA, AMANDA PEREIRA; NAGASAKO, CRISTIANE KIBUNE; FAGUNDES, JOAO JOSE; SETSUKO AYRIZONO, MARIA DE LOURDES; LEAL, RAQUEL FRANCO. Anti-TNF therapy and immunogenicity in inflammatory bowel diseases: a translational approach. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, v. 13, n. 12, p. 13916-13930, 2021. (20/01924-5)

VIDAL DE NEGREIROS, LEANDRO MINATEL; PASCOAL, LIVIA BITENCOURT; GENARO, LIVIA MOREIRA; SILVA, JULIAN FURTADO; RODRIGUES, BRUNO LIMA; CAMARGO, MICHEL GARDERE; REAL MARTINEZ, CARLOS AUGUSTO; RODRIGUES COY, CLAUDIO SADDY; SETSUKO AYRIZONO, MARIA DE LOURDES; FAGUNDES, JOAO JOSE; et al. Pouchitis: insight into the pathogenesis and clinical aspects. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, v. 14, n. 7, p. 20-pg., 2022-01-01. (20/01924-5)

CHAIM, F. H. M.; NEGREIROS, L. M. V.; STEIGLEDER, K. M.; SIQUEIRA, N. S. N.; GENARO, L. M.; OLIVEIRA, P. S. P.; MARTINEZ, C. A. R.; AYRIZONO, M. L. S.; FAGUNDES, J. J.; LEAL, R. F.. Aspects Towards the Anastomotic Healing in Crohn's Disease: Clinical Approach and Current Gaps in Research. FRONTIERS IN SURGERY, v. 9, p. 9-pg., 2022-06-24. (20/01924-5)