The aim of this study will be to evaluate the effectiveness of scaling and root planing (SRP) combined with adjunctive local and/or systemic administration of coenzyme Q10 (CQ10) for the treatment of experimental periodontitis (EP) in rats systemically treated with nicotine (NIC). One-hundred-sixty male Wistar rats will be divided into 10 groups (n=16). Throughout the experiment, animals will receive two subcutaneous injections of either 3mg/kg nicotine hemissulfate or physiological saline solution (PSS) with 12 h interval between them. These injections will initiate 30 days prior EP induction. Fifteen days after EP induction, the protocol for SRP will be performed together (or not) with local and/or systemic adjunctive CQ10 administration in animals treat with either NIC or PSS, as described: PSS-EP-SRP and NIC-EP-SRP: subgingival irrigation with PSS; PSS-EP-SRP/Q10L and NIC-EP-SRP/Q10L: subgingival irrigation with 1ml of CQ10 solution; PSS-EP-SRP/S and NIC-EP-SRP/S: daily gastric gavage with 1ml of vegetal glycerin; PSS-EP-SRP/Q10S and NIC-EP-SRP/Q10S: daily gastric gavage with 120 mg of CQ10; PSS-EP-SRP/Q10LS and NIC-EP-SRP/Q10LS: subgingival irrigation with 1ml of CQ10 solution and daily gastric gavage with 120 mg of CQ10. The euthanasia will be performed at 7 and 28 days after treatment. The specimens will be collected and processed for histopathologic, histometric and immunochemical (for of TRAP, OC, TNF-±, IL-6 and IL-10) analyzes. After normality and homoscedasticity analysis, data will be submitted to the most appropriate tests with assuming 5% of significance (pd0.05).
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