QuaMN-Ki study: quatitification with morphometry in neuroendocrine neoplasms and Ki-67 evaluation

• Grant number: 21/05917-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2021
• End date: June 30, 2022
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Vera Luiza Capelozzi
• Grantee: Maria Luiza Fernezlian de Souza
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/20403-6 - Biomolecular markers of proliferation and remodeling in acute and chronic respiratory diseases: promising therapeutic targets, AP.TEM

Abstract

The measurement of proliferative activity assumes an essential role in the gradation of several neoplasms, especially neuroendocrine ones. As an adjunct and indirect method, immunohistochemistry with Ki-67 estimation has been used ubiquitously in surgical pathology, although the tools to estimate its result are questioned. Several methods have been reported in the literature, from cell to cell count, to coarse estimation, even semi-automated methods, using computational algorithms. In the present project, we propose an inter-observer comparison between several methods already established and used in clinical practice, as well as the Ki-67 morphometry in biopsy specimens and surgical specimens with a diagnosis of neuroendocrine lung cancer, gastrointestinal and pancreatobiliary tract in order to elect the method of greater sensitivity and inter-observer replicability, as well as establishing morphometric parameters, an unprecedented data. (AU)