Clinicopathological correlation of the degree of differentiation, tetraspanin expression, viral agents and cell proliferation in a large sample of squamous cell carcinoma of the head and neck region.

• Grant number: 16/11419-0
• Support type: Regular Research Grants
• Duration: June 01, 2017 - August 31, 2019
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal researcher: Jorge Esquiche León
• Grantee: Jorge Esquiche León
• Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Assoc. researchers: Alfredo Ribeiro da Silva ; Ana Carolina Fragoso Motta ; Estela Kaminagakura Tango

Abstract

More than 90% of malignant neoplasms of the head and neck are squamous cell carcinomas (SCCs). The histopathological diagnosis of head and neck SCC (HNSCC) is made according to criteria proposed by the World Health Organization (WHO). Recent studies show variants of HNSCC, not yet recognized by the WHO, whose diagnoses often depend on immunohistochemistry (IHC), in situ hybridization (ISH) and/or molecular genetics, which need to be identified to establish appropriate therapeutic protocols. Interestingly, with apparent restriction in specific anatomic sites, the HNSCC may have different degrees of neuroendocrine and squamous differentiation, association with viral agents human (papillomavirus [HPV] and Epstein-Barr virus [EBV]), as well as express (INK4 and Cip/Kip) or suffer loss (INI-1) of proteins involved in the control of cell proliferation, justifying a comprehensive study. In addition, recent studies have highlighted the role of tetraspanins, an important component of the exosomes (nanovesicles containing proteins, DNA, RNA), which appear to be associated with tumor progression and metastasis. Therefore, it is relevant that the relation of tetraspanins/HNSCC be studied. Studies of this nature represent a valuable opportunity to elucidate the molecular mechanisms involved in the pathogenesis of HNSCC and provide data for an appropriate classification, correlating them with the survival rates and aiming to support treatment planning and prognosis. Thus, the proposal of this project will be analyzed the degree of differentiation, tetraspanin expression, viral agents and cell proliferation by IHC, ISH and molecular genetics in a large sample of HNSCC (n= 500). (AU)

Scientific publications

(27)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, LUCIANA YAMAMOTO; LOPES DOMINGUETE, MATHEUS HENRIQUE; DOMINGUETE, PAULO ROBERTO; RIBEIRO-SILVA, ALFREDO; TEIXEIRA, LUCAS RIBEIRO; LEON, JORGE ESQUICHE. Immune cell infiltration in Ectomesenchymal chondromyxoid tumor: An immunohistochemical study. Oral Oncology, v. 81, p. 112-115, JUN 2018. (11/52090-8, 16/11419-0)

SILVEIRA, HEITOR ALBERGONI; ALMEIDA, LUCIANA YAMAMOTO; WEEGE NONAKA, CASSIANO FRANCISCO; ALVES, POLLIANNA MUNIZ; RIBEIRO-SILVA, ALFREDO; LEON, JORGE ESQUICHE. Myoepithelial carcinoma with rhabdoid features in the maxillary sinus: Immunohistochemical and in situ hybridization analysis of a rare case. Oral Oncology, v. 93, p. 116-119, JUN 2019. (18/12734-2, 16/02713-2, 16/11419-0)

FERRISSE, TULIO M.; ALMEIDA, LUCIANA Y.; SILVEIRA, HEITOR A.; DE OLIVEIRA, ANALU B.; BUFALINO, ANDREIA; LEON, JORGE E.. Benign atypical intralymphatic CD30+lymphoid proliferation with activated regulatory T-cell phenotype in the oral cavity. JOURNAL OF CUTANEOUS PATHOLOGY, v. 46, n. 11, JULY 2019. (18/12734-2, 16/11419-0, 16/02713-2)

DOS SANTOS, JESSICA LUANA; DE ALMEIDA MILANI ALTEMANI, ALBINA MESSIAS; TRIVELLATO, ALEXANDRE ELIAS; SVERZUT, CASSIO EDVARD; ALMEIDA, LUCIANA YAMAMOTO; TEIXEIRA, LUCAS RIBEIRO; RIBEIRO-SILVA, ALFREDO; LEON, JORGE ESQUICHE. Intraoral Pigmented Low-Grade Adenocarcinoma, Not Otherwise Specified: Case Report and Immunohistochemical Study. Head and Neck Pathology, v. 12, n. 4, p. 610-618, DEC 2018. (16/11419-0, 11/52090-8)

MARTINS PANUCCI, BEATRIZ ZAMBONI; SILVA, EVANIO VILELA; ARTERO AREVALO, RAFAEL HUMBERTO; RIVERA MIRANDA, RICARDO ERNESTO; MARIA CUADRA ZELAYA, FLORENCE JUANA; SILVEIRA, HEITOR ALBERGONI; LEON, JORGE ESQUICHE. Chondromyxoid fibroma affecting the maxilla in a 1-year-old child: Immunohistochemical analysis and literature review. Oral Oncology, v. 124, JAN 2022. (16/11419-0)

ROCHA, AUDREY F. L.; MIOTTO, LARISSA N.; FERRISSE, TULIO M.; SILVEIRA, HEITOR A.; ALMEIDA, LUCIANA Y.; BUFALINO, ANDREIA; NAVARRO, CLAUDIA M.; LEON, JORGE E.. Low-fat and fat-free spindle cell lipomas in the oral cavity: Immunohistochemical analysis and review of the literature. JOURNAL OF CUTANEOUS PATHOLOGY, v. 46, n. 10, p. 778-783, OCT 2019. (18/12734-2, 16/11419-0)

RODRIGO N. SILVA; HEITOR A. SILVEIRA; LUCIANA Y. ALMEIDA; CARLA N. B. COLTURATO; ALEXANDRE ELIAS TRIVELLATO; CASSIO E. SVERZUT; JORGE E. LEÓN. Carcinoma cuniculado gengival mimetizando um processo inflamatório/reativo. Jornal Brasileiro de Patologia e Medicina Laboratorial, v. 55, n. 5, p. 498-505, 2019-11-11. (16/11419-0, 16/02713-2, 18/12734-2)

TEIXEIRA, LUCAS R.; ALMEIDA, LUCIANA Y.; SILVA, RODRIGO N.; MESQUITA, ANA T. M.; COLTURATO, CARLA B. N.; SILVEIRA, HEITOR A.; DUARTE, ANDRESSA; RIBEIRO-SILVA, ALFREDO; LEON, JORGE E.. Young and elderly oral squamous cell carcinoma patients present similar angiogenic profile and predominance of M2 macrophages: Comparative immunohistochemical study. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, v. 41, n. 12, p. 4111-4120, DEC 2019. (18/12734-2, 16/11419-0, 17/02502-4, 16/02713-2)

BERTASSO, A. S.; LEON, J. E.; SILVA, R. A. B.; SILVA, L. A. B.; DE QUEIROZ, A. M.; PUCINELLI, C. M.; ROMUALDO, P. C.; NELSON-FILHO, P.. Immunophenotypic quantification of M1 and M2 macrophage polarization in radicular cysts of primary and permanent teeth. International Endodontic Journal, v. 53, n. 5, DEC 2019. (16/11419-0)

DA MOTTA, RAPHAEL J. G.; ALMEIDA, LUCIANA YAMAMOTO; VILLAFUERTE, KELLY R. V.; RIBEIRO-SILVA, ALFREDO; LEON, JORGE E.; TIRAPELLI, CAMILA. FOXP3+and CD25+cells are reduced in patients with stage IV, grade C periodontitis: A comparative clinical study. JOURNAL OF PERIODONTAL RESEARCH, v. 55, n. 3, DEC 2019. (16/11419-0, 13/08589-3, 16/02713-2)

NEVES-SILVA, RODRIGO; ALMEIDA, LUCIANA Y.; SILVEIRA, HEITOR A.; COLTURATO, CARLA B. N.; DUARTE, ANDRESSA; FERRISSE, TULIO M.; SILVA, V, EVANIO; VANZOLIN, BARBARA F.; BUFALINO, ANDREIA; RIBEIRO-SILVA, ALFREDO; et al. SMARCB1 (INI-1) and NUT immunoexpression in a large series of head and neck carcinomas in a Brazilian reference center. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, NOV 2019. (18/12734-2, 16/11419-0, 16/02713-2)

FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; PALACON, MARIANA PARAVANI; DA SILVEIRA, HEITOR ALBERGONI; SGAVIOLI MASSUCATO, ELAINE MARIA; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. Immunohistochemical evaluation of Langerhans cells in oral lichen planus and oral lichenoid lesions. ARCHIVES OF ORAL BIOLOGY, v. 124, APR 2021. (16/11419-0, 17/01438-0)

FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; PALACON, MARIANA PARAVANI; SILVA, EVANIO VILELA; SGAVIOLI MASSUCATO, ELAINE MARIA; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. The role of CD68+and CD163+macrophages in immunopathogenesis of oral lichen planus and oral lichenoid lesions. Immunobiology, v. 226, n. 3, MAY 2021. (20/07110-0, 16/11419-0, 17/01438-0, 18/18440-0)

CAMILA DE OLIVEIRA BARBEIRO; ROBERTO HENRIQUE BARBEIRO; HEITOR ALBERGONI DA SILVEIRA; LUCIANA YAMAMOTO DE ALMEIDA; JORGE ESQUICHE LEÓN; ANDREIA BUFALINO. Maxillary dentigerous cyst showing squamous odontogenic tumor-like proliferation: surgical approach and literature review. Autopsy and Case Reports, v. 11, 2021-07-02. (16/11419-0, 17/01438-0, 18/04954-2, 18/12734-2)

YACARINI PAREDES, SILVIA ELENA; ALMEIDA, LUCIANA YAMAMOTO; TREVISAN, GLAUCE LUNARDELLI; JIMENEZ POLANCO, XIOMARA BEATRIZ; SILVEIRA, HEITOR ALBERGONI; SILVA, EVANIO VILELA; BEZERRA SEGATO, RAQUEL ASSED; BEZERRA DA SILVA, LEA ASSED; CHAHUD, FERNANDO; LEON, JORGE ESQUICHE. Immunohistochemical characterization of immune cell infiltration in paediatric and adult Langerhans cell histiocytosis. Scandinavian Journal of Immunology, v. 92, n. 6, AUG 2020. (16/11419-0)

ORTEGA, ROSE MARA; BUFALINO, ANDREIA; ALMEIDA, LUCIANA YAMAMOTO; NAVARRO, CLAUDIA MARIA; TRAVASSOS, DAPHINE CAXIAS; FERRISSE, TULIO MORANDIN; CARLOS, ROMAN; LEON, JORGE ESQUICHE. Synchronous Polymorphous Adenocarcinoma and Canalicular Adenoma on the Upper Lip: An Unusual Presentation and Immunohistochemical Analysis. Head and Neck Pathology, v. 12, n. 1, p. 145-149, MAR 2018. (11/52090-8, 16/11419-0)

SILVA, EVANIO VILELA; ALMEIDA, LUCIANA YAMAMOTO; BORTOLETTO, KAREN CRISTINE; QUERO, ISABELA BARBOSA; JACOMINI, FERNANDA CAROLINA; BENEVENUTO DE ANDRADE, BRUNO AUGUSTO; SILVEIRA, HEITOR ALBERGONI; DUARTE, ANDRESSA; PETEAN, FLAVIO CALIL; ROCHA, EDUARDO MELANI; et al. Focal lymphocytic sialadenitis and ectopic germinal centers in oral reactive lesions and primary Sjogren's syndrome: a comparative study. RHEUMATOLOGY INTERNATIONAL, JUL 2021. (16/02713-2, 16/14636-2, 18/12734-2, 16/11419-0)

TAKAHAMA JR, ADEMAR; DE LIMA, HELITON GUSTAVO; ITO, FABIO AUGUSTO; RIBEIRO-SILVA, ALFREDO; LEON, JORGE ESQUICHE. Epstein-Barr Virus-Positive Mucocutaneous Ulcer Mimicking Peri-Implantitis in a Patient With Systemic Lupus Erythematosus. JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, v. 77, n. 5, p. 977-984, MAY 2019. (16/11419-0, 16/02713-2)

YACARINI PAREDES, SILVIA ELENA; BEZERRA SEGATO, RAQUEL ASSED; MOREIRA, LEILA DAHER; MOREIRA, ALCIDES; DIAZ SERRANO, KRANYA VICTORIA; RODRIGUES, CLARISSA TELES; ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE. Dentoalveolar Abscesses Not Associated with Caries or Trauma: A Diagnostic Hallmark of Hypophosphatemic Rickets Initially Misdiagnosed as Hypochondroplasia. Head and Neck Pathology, v. 12, n. 4, p. 604-609, DEC 2018. (16/11419-0, 11/52090-8)

ALMEIDA, LANA KEI YAMAMOTO; JABUR, ROBERTO OLIVEIRA; SILVEIRA, HEITOR ALBERGONI; POLANCO, XIOMARA BEATRIZ JIMENEZ; SILVA, RAQUEL ASSED BEZERRA DA; RIBEIRO-SILVA, ALFREDO; LEON, JORGE ESQUICHE. Gingival ossifying myopericytoma in a pediatric patient: Immunohistochemical analysis and literature review. Oral Oncology, v. 107, AUG 2020. (16/11419-0)

POLANCO, X. B. J.; BERTASSO, A. S.; SILVEIRA, H. A.; YAMAMOTO DE ALMEIDA, L.; ALMEIDA, L. K. Y.; DA SILVA, R. A. B.; DA SILVA, L. A. B.; DE ROSSI, A.; NELSON-FILHO, P.; LEON, J. E.. IgG4-positive plasma cells are more often detected in chronic periapical lesions arising from permanent rather than primary teeth. International Endodontic Journal, v. 54, n. 5, JAN 2021. (16/02713-2, 16/11419-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; SILVEIRA, HEITOR ALBERGONI; SILVA, EVANIO VILELA; BUFALINO, ANDREIA; LEON, JORGE ESQUICHE; BRIGHENTI, FERNANDA LOURENCAO. Intraoral atypical lentiginous melanocytic lesion in a pediatric patient. Oral Oncology, v. 112, JAN 2021. (16/11419-0, 18/18440-0)

SILVA, EVANIO V.; SILVEIRA, HEITOR A.; MESQUITA, ANA T. M.; ALMEIDA, LUCIANA Y.; RIBEIRO-SILVA, ALFREDO; LEON, JORGE E.. Elastofibromatous lesion as a differential diagnosis of white lesions of the oral mucosa. JOURNAL OF CUTANEOUS PATHOLOGY, v. 47, n. 3, p. 307+, MAR 2020. (18/12734-2, 16/11419-0, 16/02713-2)

SILVEIRA, HEITOR ALBERGONI; ALMEIDA, LUCIANA YAMAMOTO; LOPES DOMINGUETE, MATHEUS HENRIQUE; PEREIRA GRACIANO, KAMILA PRADO; BUFALINO, ANDREIA; LEON, JORGE ESQUICHE. Intraoral epidermoid cyst with extensive elastofibromatous changes: an unusual finding. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, v. 23, n. 4, p. 493-497, DEC 2019. (18/12734-2, 16/11419-0)

SILVEIRA, HEITOR ALBERGONI; SOUSA, LUCAS MOURA; SILVA, EVANIO VILELA; ALMEIDA, LANA KEI YAMAMOTO; SVERZUT, CASSIO EDVARD; TRIVELLATO, ALEXANDRE ELIAS; LEON, JORGE ESQUICHE. Primary intraosseous CD9-positive B-cell lymphoblastic lymphoma of the maxilla affecting a pediatric patient: Immunohistochemical and in situ hybridization analysis. Oral Oncology, v. 108, SEP 2020. (18/12734-2, 16/11419-0)

LUCIANA YAMAMOTO ALMEIDA; HEITOR ALBERGONI SILVEIRA; EVÂNIO VILELA SILVA; CAMILA DE OLIVEIRA BARBEIRO; JOAQUIM AUGUSTO DE PAULA; ANDREIA BUFALINO; ALFREDO RIBEIRO-SILVA; JORGE ESQUICHE LEÓN. EBV-negative lymphoepithelial-like carcinoma of the lower lip. Autopsy and Case Reports, v. 10, n. 1, 2020-10-21. (16/11419-0, 16/02713-2, 18/12734-2)

SILVEIRA, HEITOR ALBERGONI; TORAL-RIZO, VICTOR HUGO; LARA-CARRILLO, EDITH; DOMINGUETE, MATHEUS HENRIQUE LOPES; SILVA, EVANIO VILELA; RIBEIRO-SILVA, ALFREDO; BUFALINO, ANDREIA; ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE. Spongiotic hyperplasia of the oral mucosa: case series and immunohistochemical analysis. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, AUG 2021. (17/01438-0, 16/11419-0, 16/02713-2, 18/12734-2)