In situ effect of an experimental toothpaste containing casein phosphopeptide - amorphous calcium phosphate and sodium trimetaphosphate on enamel demineralization

• Grant number: 21/06408-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2021
• End date: February 28, 2023
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Marcelle Danelon
• Grantee: Eduarda Martins Fontes Cantarella de Almeida
• Host Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil

Abstract

The aim of the present study will be to evaluate, in situ, the ability of a new toothpaste formulation containing fluoride (F), sodium trimetaphosphate (TMP) and amorphous casein-calcium phosphopeptide (CPP-ACP) to reduce demineralization of bovine dental enamel. Bovine enamel blocks (n=192) will be selected by initial surface hardness (HS). Then twelve volunteers (n=12) will wear palatal devices containing 4 bovine enamel blocks for 7 days in 5 experimental phases with the respective toothpastes: 1) Placebo (no F-CPP-ACP-TMP), 2) 1100 ppm F (1100F), 3) 1100 ppm F associated with 3% TMP (1100F-TMP), 4) 1100 ppm F associated with 10% CPP-ACP (MI Paste One®) and 5) 1100 ppm F associated with 10% CPP-ACP and 3% TMP (1100F-CPP-ACP-TMP). The cariogenic challenges will be produced by using a 30% sucrose solution, 6x/day. After 7 days the final surface hardness (SHf) and the percentage of surface hardness loss (%SH) will be determined. The most appropriate statistical test for data distribution will be used, through the statistical software program Sigmaplot© for Windows version 12.0, with significance at the 5% level.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

GONCALVES, FRANCYENNE MAIRA CASTRO; QUINTEIRO, JULIA PAIAO; HANNIG, CHRISTIAN; DE ALMEIDA, EDUARDA MARTINS FONTES CANTARELLA; DELBEM, ALBERTO CARLOS BOTAZZO; CANNON, MARK LLOYD; DANELO, MARCELLE. In situ remineralization of enamel caries lesions with a toothpaste supplemented with fluoride, amorphous calcium phosphate casein phosphopeptide and trimetaphosphate. Journal of Dentistry, v. 155, p. 7-pg., 2025-04-01. (21/06408-8)

MEIRA, MARIA FERNANDA CAVALCANTE; FERNANDES, GABRIELA LEAL PERES; CAMARGO, ANDREA SIMONE STUCCHI DE; RAVARO, LEANDRO PIAGGI; ARAI, MARYLYN SETSUKO; NAVARRO, MARIA FIDELA DE LIMA; BRIGHENTI, FERNANDA LOURENCAO; DE OLIVEIRA, ANALU BARROS; DANELON, MARCELLE. Modification of restorative glass ionomer cement with zinc oxide nanoparticles and calcium glycerophosphate microparticles: in vitro assessment of mechanical properties and antimicrobial activity. Journal of Applied Oral Science, v. 33, p. 14-pg., 2025-01-01. (21/06408-8, 13/07793-6)

GONCALVES, FRANCYENNE MAIRA CASTRO; ALMEIDA, EDUARDA MARTINS FONTES CANTARELLA DE; HANNIG, CHRISTIAN; QUINTEIRO, JULIA PAIAO; DELBEM, ALBERTO CARLOS BOTAZZO; CANNON, MARK LLOYD; DANELON, MARCELLE. Biofilm modulation and demineralization reduction after treatment with a new toothpaste formulation containing fluoride, casein phosphopeptide-amorphous calcium phosphate, and sodium trimetaphosphate: In situ study. Dental Materials, v. 40, n. 12, p. 8-pg., 2024-12-10. (21/06408-8)