Characterization of multidrug-resistant Brevundimonas isolated from intensive-care-unit patient sample using whole-genome sequencing

• Grant number: 21/08423-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2021
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Maria Cristina da Silva Pranchevicius
• Grantee: Gabriela Guerrera Soares
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Gram-negative bacteria, such as Brevundimonas vesicularis and Brevundimonas diminuta, have been reported as nosocomial, opportunistic, and multidrug resistant pathogens. Brevundimonas can cause several infections; however, there is a lack of studies concerning these bacteria. In this study, we aim to sequence the whole genome of a Multidrug-Resistant (MDR) Brevundimonas strain, isolated from a patient in a Neonatal Intensive Care Unit. The strain was previously analyzed by the automated Vitek-2 system and presented a multidrug-resistant profile, yet these phenotyping analyses were not sufficient to identify the species. Accordingly, we intend to investigate the identity of the species, the presence of antibiotic resistance and virulence genes, together with a comparative genomic analysis with other Brevundimonas species, taking into consideration the essentiality of the genes, biological processes, and functionality of the strain. Studies regarding the genomic and molecular characterization of multidrug-resistant resistant bacterial strains are still incipient in our country and the link between clonal diversity of strains, resistance genes, and virulence factors is very complex. Supplementary molecular studies are extremely important to understand how the presence of virulence factors is affected by or related to bacterial resistance factors. To the best of our knowledge, this is the first study on the genomic profiling of Brevundimonas isolated from neonatal ICU in Brazil. Therefore, our results will contribute to describing the spread of these genes, which will provide a positive impact in both clinical practices and basic research. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES, GABRIELA GUERRERA; CAMPANINI, EMELINE BONI; FERREIRA, ROUMAYNE LOPES; DAMAS, MARCELO SILVA FOLHAS; RODRIGUES, SAULO HENRIQUE; CAMPOS, LESLIE CAMELO; GALVAO, JUCIMARIA DANTAS; FUENTES, ANDREA SOARES DA COSTA; FREIRE, CAIO CESAR DE MELO; MALAVAZI, IRAN; et al. Brevundimonas brasiliensis sp. nov.: a New Multidrug-Resistant Species Isolated from a Patient in Brazil. MICROBIOLOGY SPECTRUM, v. 11, n. 3, p. 21-pg., 2023-04-17. (18/20697-0, 20/11964-4, 21/08423-4, 18/24213-7)

DAMAS, MARCELO SILVA FOLHAS; FERREIRA, ROUMAYNE LOPES; CAMPANINI, EMELINE BONI; SOARES, GABRIELA GUERRERA; CAMPOS, LESLIE CAMELO; LAPREGA, PEDRO MENDES; DA COSTA, ANDREA SOARES; FREIRE, CAIO CESAR DE MELO; PITONDO-SILVA, ANDRE; CERDEIRA, LOUISE TEIXEIRA; et al. Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil. FRONTIERS IN MEDICINE, v. 9, p. 20-pg., 2022-07-28. (18/24213-7, 21/08423-4, 20/11964-4, 18/20697-0, 21/00425-8)