Evaluation of the antitumoral effect of latex of janauba (Synadenium grantii) in 3d cultivation of canine oral melanoma cells

• Grant number: 21/01390-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2021
• End date: July 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Carlos Eduardo Fonseca Alves
• Grantee: Isabela Aguiar de Castro
• Host Institution: Vice-Reitoria de Pesquisa e Pós-Graduação. Universidade Paulista (UNIP). São Paulo , SP, Brazil

Abstract

Melanoma is a neoplasm that occurs from the mutation of melanocytes (melanin-producing cells present in the epidermis), with oral melanoma being the most common oral malignant neoplasm in dogs, with a more severe prognosis than tumors occurring in the skin. It is a locally aggressive tumor, it makes regional and also more distant metastases, via the lymphatic system, through the regional lymph nodes, or via hematogen, reaching organs such as the lung. Treatment is based on surgical excision, chemotherapy or radiotherapy, but the efficiency of treatments is low and the prognosis for survival over one year is no more than 10%. Therefore, the continuous study of this neoplasm is extremely important, looking for new alternatives to improve the prognosis and expectation of survival of these animals. The present study aims to compare the antitumor effect between doxorubicin, the chemotherapy of choice in the fight against oral canine melanoma, and the latex of janauba, a plant widespread in Brazil and popularly known for its effects against cancer. For this, the samples of melanoma will be analyzed with four different concentrations of doxorubicin (0.5 ¼M, 1 ¼M, 2 ¼M and 2.5 ¼M) and four different concentrations of janauba latex (1mg/ml, 3 mg/ml e 6 mg/ml). The effect of chemotherapy in combination with the plant's latex will be analyzed using 96-well plates and concentrations will be tested in 24, 48 and 72 hours. After testing in 2D culture, doses will be tested on 3D spheroids. With this study it is expected to find alternative ways to treat canine oral melanoma, promoting natural therapies that can generate results equal or even better than with the chemotherapy of choice.(AU)