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Study of expression of annexin A1 and NLRP3 inflammasome in atopic Dermatitis

Grant number: 21/04071-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2021
Effective date (End): August 31, 2022
Field of knowledge:Biological Sciences - Morphology - Histology
Principal Investigator:Cristiane Damas Gil
Grantee:Maria Eduarda de Castro
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Annexin A1 (ANXA1) is an anti-inflammatory protein and has a decisive role in the inflammatory response through the control of leukocyte migration, the release of pro-inflammatory cytokines, and the phagocytosis of macrophages. In allergic processes, this protein also regulates the production of IgE, the release of Th2 profile cytokines, and mast cell degranulation. In addition, ANXA1 regulates the activation of the NLRP3 inflammasome in inflammatory cells, but this relationship has not been explored in cells related to allergic diseases. Thus, this study will investigate the expression patterns of mRNA and ANXA1 proteins and NLRP3 inflammasome in the skins of mice and patients with AD. In the experimental model of AD induced by ovalbumin, the skins of Balb/c mice will be evaluated under the following experimental conditions (n = 6 animals/group): Sham (control), and DA (atopic dermatitis); obtained from the studies by Corrêa et al., J. Mol. Medicine, 2017 (doi: 10.1007/ s00109-017-1566-9). Under these conditions, we will analyze: I) histopathology of the skin; II) localization and possible modulation of the expression of ANXA1, and NLRP3 by means of immunohistochemistry, and III) possible co-localization of ANXA1 and NLRP3 by immunofluorescence. In the publicly available transcriptomes of the Gene Expression Omnibus (GEO) repository, we will assess the transcriptional levels of ANXA1, and NLRP3 using skin studies of patients with AD and controls. The results will contribute to a better understanding of the role of ANXA1 and NLRP3 in AD, as well as possible therapeutic targets for inflammatory skin processes. (AU)

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