The effects of Plexomin L-Mn on bovine satellite cell differentiation and GLUT-4 abundance in a cell culture model

• Grant number: 21/08116-4
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: October 01, 2021
• End date: January 31, 2022
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Danilo Domingues Millen
• Grantee: Luana Doretto Felizari
• Supervisor: Bradley Joseph Johnson
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil
• Institution abroad: Texas Tech University (TTU), United States
• Associated to the scholarship: 19/26951-8 - Effects of nutritional protocols for marbling on meat traits and beef quality of Nellore cattle finished in feedlot, BP.MS

Abstract

The objective of this project will be conduct dose titration studies with Plexomin L-Mn to determine the effects of added Mn and lysine on satellite cell GLUT- 4 concentrations, differentiation status and myosin heavy chain expression (MHC I). In this project, the semimembranosus from 2 steers with 16 months, will be used to isolate bovine primary satellite cell (BSC). Myogenic differentiation will be induced using 3% horse serum upon cultured BSC with increasing doses (0, 0.1 ¼M, 1.0 ¼M, 10 ¼M, or 100 ¼M) of Mn, and the treatments will be done in triplicate. After 96 h of incubation, cells will be harvested and used to measure the gene expression of AMPK±, ²1-AR, ²2-AR, IGF-1, MHC I, MHC IIa, and MHC IIx, glucose transporter 4 (GLUT4) and relative to ribosomal protein subunit 9 (RPS-9). Data will be analyzed by PROC MIXED of SAS (2003). (AU)