Molecular characterization of Cryptosporidium spp. in donkeys and mules

• Grant number: 21/04997-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2021
• End date: September 30, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Katia Denise Saraiva Bresciani
• Grantee: Lívia Cavalari Morales
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This research project aims to carry out the molecular characterization of Cryptosporidium spp. in donkeys and mules. A total of 100 fecal samples will be collected and examined using the negative coloring of the malachite green staining technique so that the oocysts of the parasite can be detected. Subsequently, all samples will be subjected to DNA extraction with subsequent molecular characterization by polymerase chain reaction for amplification of fragments of the 18S subunit of the rRNA and sequencing of the amplified fragments, followed by a subtype using the gp60 gene. The results will be analyzed using descriptive statistics and inferential analysis (Fisher's Exact Test) to verify the association between the presence and absence of the protozoan with each of the studied variables. (AU)