Analysis of the rev-erb-alpha protein interactome through immunoprecipitation of target protein and identification of possible ligands by mass spectrometry in cell culture

• Grant number: 21/08692-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2021
• End date: September 30, 2025
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Adelino Sanchez Ramos da Silva
• Grantee: Vitor Rosetto Muñoz
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 19/11820-5 - Nr1d1 function on the aging-associated Sarcopenia, AP.TEM
• Associated scholarship(s): 22/05957-0 - The role of circadian clock-related genes in skeletal muscle insulin resistance and type 2 diabetes, BE.EP.PD

Abstract

The analysis from the scientific literature regarding the proteins related to rev-erv-alpha revealed important interactions with circadian rhythm markers, such as, CLOCK (clock circadian regulator) and OPHN1 (oligophrenin 1) in the neuronal context, and RORA (RAR related orphan receptor A) and NCOR1 (nuclear receptor corepressor 1) in the transcription context, modulating the expression of proteins related to metabolism, such as PPARG4 (peroxisome proliferator activated receptor gamma). However, a few studies emphasized the role of rev-erb-alpha in the skeletal muscle and which proteins and molecular pathways rev-erb-alpha are involved. Based on our preliminary data using bioinformatic analysis (figure 2A-2C), rev-erb-alpha protein could be associated with other cellular and molecular events not yet investigated in the skeletal muscle. Professor Dr. Fernando Moreira Simabuco (associated researcher of the thematic project) have been using interactome analysis of cellular proteins to reveal new signaling pathways and functions of already known proteins. Therefore, to identify new proteins interactions and molecular pathways related to rev-erb-alpha in the context of myogenesis and muscle metabolism, subproject 3 will analyze the rev-erb-alpha interctoma in skeletal muscle cells C2C12. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUNOZ, VITOR ROSETTO; BOTEZELLI, JOSE DIEGO; GASPAR, RAFAEL CALAIS; DA ROCHA, ALISSON L.; VIEIRA, RENAN FUDOLI LINS; CRISOL, BARBARA MOREIRA; BRAGA, RENATA ROSSETO; SEVERINO, MATHEUS BRANDEMARTE; NAKANDAKARI, SUSANA CASTELO BRANCO RAMOS; ANTUNES, GABRIEL CALHEIROS; et al. Effects of short-term endurance and strength exercise in the molecular regulation of skeletal muscle in hyperinsulinemic and hyperglycemic Slc2a4(+/-) mice. CELLULAR AND MOLECULAR LIFE SCIENCES, v. 80, n. 5, p. 16-pg., 2023-05-01. (16/18488-8, 21/08692-5, 15/26000-2, 19/11820-5, 20/13443-1, 18/20872-6)

PINTO, ANA P.; MUNOZ, VITOR R.; DA ROCHA, ALISSON L.; ROVINA, RAFAEL L.; FERRARI, GUSTAVO D.; ALBERICI, LUCIANE C.; SIMABUCO, FERNANDO M.; TEIXEIRA, GIOVANA R.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; et al. IL-6 deletion decreased REV-ERB alpha protein and influenced autophagy and mitochondrial markers in the skeletal muscle after acute exercise. FRONTIERS IN IMMUNOLOGY, v. 13, p. 13-pg., 2022-10-13. (21/06291-3, 21/08693-1, 19/11820-5, 17/12765-2, 19/21709-4, 21/08692-5, 20/13443-1, 18/14818-9)

MUNOZ, VITOR ROSETTO; VIEIRA, RENAN FUDOLI LINS; KATASHIMA, CARLOS KIYOSHI; GASPAR, RAFAEL CALAIS; LINO, MARSEL; TROMBETA, JOICE CRISTINA DOS SANTOS; DUFT, RENATA GARBELLINI; AZEVEDO MACEDO, ANA PAULA; DA SILVA, ADELINO SANCHEZ RAMOS; ROPELLE, EDUARDO ROCHETE; et al. Rho-Kinase Is Differentially Expressed in the Adipose Tissue of Rodent and Human in Response to Aging, Sex, and Acute Exercise. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v. 79, n. 3, p. 10-pg., 2024-02-20. (20/13443-1, 21/08692-5, 18/20872-6, 16/18488-8, 19/11820-5, 15/26000-2)

MUNOZ, V. R.; GASPAR, R. C.; MANCINI, M. C. S.; DE LIMA, R. D.; VIEIRA, R. F. L.; CRISOL, B. M.; ANTUNES, G. C.; TROMBETA, J. C. S.; BONFANTE, I. L. P.; SIMABUCO, F. M.; et al. Short-term physical exercise controls age-related hyperinsulinemia and improves hepatic metabolism in aged rodents. Journal of Endocrinological Investigation, v. N/A, p. 13-pg., 2022-11-01. (16/18488-8, 19/11820-5, 18/20872-6, 21/08692-5, 20/13443-1, 15/26000-2)

PINTO, ANA P.; MUNOZ, VITOR R.; TAVARES, MARIA EDUARDA A.; DOS SANTOS, JONATHAS R.; REBELO, MACARIO A.; ALBERICI, LUCIANE C.; SIMABUCO, FERNANDO M.; TEIXEIRA, GIOVANA R.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; et al. Combined physical exercise reverses the reduced expression of Bmal1 in the liver of aged mice. Life Sciences, v. 312, p. 12-pg., 2023-01-01. (21/08693-1, 21/08692-5, 20/13443-1, 21/06291-3, 19/11820-5)

NETO, IVO VIEIRA DE SOUSA; PINTO, ANA PAULA; ANDRADE, ROSANGELA VIEIRA DE; DE SOUZA, FABIANE HIRATSUKA VEIGA; DE SOUZA, PAULO EDUARDO NARCIZO; ASSIS, VICTORIA; TIBANA, RAMIRES ALSAMIR; NEVES, RODRIGO VANERSON PASSOS; ROSA, THIAGO SANTOS; PRESTES, JONATO; et al. Paternal exercise induces antioxidant defenses by α-Klotho/Keap1 pathways in the skeletal muscle of offspring exposed to a high fat-diet without changing telomere length. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 134, p. 15-pg., 2024-09-23. (21/06291-3, 21/08693-1, 21/08692-5, 21/14233-3, 19/11820-5)