Injectable systems with controlled release of simvastatin: development strategy for dentin regeneration

• Grant number: 21/11090-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2021
• Effective date (End): November 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Diana Gabriela Soares dos Passos
• Grantee: Beatriz Rodrigues Carlucci
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated research grant: 16/15674-5 - Association of tissue engineering techniques for mineralized tissue regeneration under degenerative inflammatory stimulus: analysis on 3D-culture perfusion bioreactor and animal inflammatory models, AP.JP

Abstract

The development of injectable biomaterials, capable of acting as a framework for tissue neo-deposition, presents itself as an innovative proposal within the current concepts of Regenerative Dentistry. Thus, this project presents a strategy for the development of an injectable system based on gelatin-collagen hydrogel (HGel) associated with chitosan (CH) microspheres (MS) loaded with a drug with pleiotropic effect, simvastatin (SV), aiming at the creation of a controlled release system at bioactive dosages for dentin regeneration. The MS-CH-SV have already been developed and characterized. On the first stage of the project, the biostimulation of human dental pulp cells (HDPCs) mediated by MS-CH-SV components will be assessed to determine the percentage of incorporation into HGel. Then the HGel will be produced containing or not MS-CH-SV in predetermined concentrations, and its injectability and bioactivity onto HDPCs will be tested. (AU)