Effects of Limosilactobacillus reuteri with vitamin K2 in induced osteoporosis in mice: microtomographic study

• Grant number: 21/09726-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2022
• End date: February 28, 2023
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ana Lia Anbinder
• Grantee: Luisa Souza Battistelli
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Osteoporosis is an osteometabolic disease characterized by an imbalance between bone deposition and resorption, constituting an important public health problem. The relation between probiotics and bone metabolism has received attention as a possible form of therapy for osteoporosis. The association of probiotics with vitamin K is very interesting since the benefic ways of both may be interconnected. Vitamin K is related to increased bone mineral density, stimulation of osteoblastogenesis, and inhibition of osteoclastogenesis and acts as a co-factor for osteocalcin gamma-carboxylation, important for bone mineralization. In this study, the effects of Limosilactobacillus reuteri (current nomenclature of Lactobacillus Reuters) associated with vitamin K2 on protection against bone loss induced by ovariectomy in female mice will be evaluated. The animals will be divided into five groups: group 1, control; group 2, ovariectomy; group 3, ovariectomy + L. reuteri; group 4, ovariectomy + vitamin K2; group 5, ovariectomy + L. reuteri + vitamin K2. Treatment will begin one week after ovariectomy and will last 4 weeks when euthanasia will be performed. The data obtained will be submitted to the most convenient statistical test, at the 5% level. The administration of the probiotic is expected to lead to a reduction in bone loss stimulated by ovariectomy. It is also expected that the addition of vitamins to the probiotic can increase its anabolic effect already demonstrated in the literature, offering a low-cost nutritional alternative for the control of postmenopausal osteoporosis.(AU)