The effect of glucocorticoid and/or insulin treatment in the exosomal miRNAs profile of different adipocytes

• Grant number: 22/01363-9
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: May 01, 2022
• End date: April 30, 2023
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Fabio Bessa Lima
• Grantee: Rafael Barrera Salgueiro
• Supervisor: Carl Ronald Kahn
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Harvard University, Boston, United States
• Associated to the scholarship: 19/19669-4 - Alterations in adipogenesis and apoptosis in different adipose territories induced by the chronic iatrogenic hypercortisolism, BP.PD

Abstract

The adipose tissue is a heterogeneous organ, and each cell subtypes have different metabolic profiles. It is well known that adipocytes are an important source of circulating exosomal miRNAs which could regulate a distant tissue's metabolism, performing a crucial mechanism in cell-to-cell communication. Our group has already identified that in vivo glucocorticoid treatment deregulates brown adipose tissue's miR-21, miR-708, miR-212 and miR-146a expression, and it also contrasted with the these miRNAs concentration found in serum, which could indicate that other tissues might be consuming these miRNAs in the high glucocorticoid induced metabolic disorder. Since the bloodstream miRNA profile is a dynamic process of release and degradation of these molecules, this study aims to identify all the exosomal miRNAs coming from every fat cell type in vitro and to determine if their secretions are modified by insulin and/or glucocorticoid treatment. The idea is to extract the small extracellular vesicles carrying miRNAs after insulin and/or glucocorticoid to analyze the miRNA expression profile. With miRNA secretion profile, our aim is to determine the adipocytes' contributions derived from different fat depots in the crosstalk between different types of cells. (AU)