Expression of the pSAMBA-GUS-GFP reporter gene at different stages of development of the Micro-tom tomato

• Grant number: 21/13844-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: May 31, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Agreement: Max Planck Society for the Advancement of Science
• Principal Investigator: Nubia Barbosa Eloy
• Grantee: Gabriela de Fátima Cia
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 17/10333-8 - APC as an entry point to study small molecule regulation of the cell cycle, AP.JP

Abstract

The Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle proteins by the 26S proteasome. The spatio-temporal degradation of mitotic cyclins and securins ensures the correct onset of the cell cycle phases and exit from the cell division program, respectively. The identification of genes encoding APC/C subunits in Arabidopsis thaliana suggests that the complex has other specific functions during plant development, such as embryogenesis, gametogenesis, growth regulation, hormone signaling and symbiotic interactions. Despite extensive research on APC/C, only a few components have been characterized in plants. One of these proteins, SAMBA, proved to be a highly promising candidate in Arabidopsis with regard to increasing productivity, through production of larger leaves, roots and seeds. In this project we aim to characterize the expression of the pSAMBA-GUS-GFP reporter gene at different stages of development of the Micro-tom tomato. (AU)