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Optimization of carbendazim fungicide detection via SERS scattering: performance of benchtop vs portable equipment and liquid vs solid substrates

Grant number: 22/04020-5
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): July 01, 2022
Status:Discontinued
Field of knowledge:Engineering - Materials and Metallurgical Engineering
Principal Investigator:Carlos José Leopoldo Constantino
Grantee:Tatiana Aparecida de Oliveira
Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
Associated research grant:18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
Associated scholarship(s):23/04786-0 - Development of paper-based SERS substrates for pesticide detection, BE.EP.MS

Abstract

Pesticides are used in plantations to kill insects, larvae, fungi, and weeds that can spread diseases in plants, making it impossible for vegetation to grow. On the other hand, the use of pesticides outside the conditions established by technical rules can result in negative impacts on the environment and human and animal health. In this context, research on pesticide detection has been growing to identify, monitor, and understand their impacts. This project aims to optimize the detection process of the fungicide carbendazim through Surface-enhanced Raman Spectroscopy (SERS). Carbendazim is a fast-absorbing fungicide, legalized in Brazil, with protective and curative action, used in cotton, rice, beans, corn, and soybean crops. Our research group has previous experience in detecting carbendazim and, for this reason, it was chosen as the target molecule. In the optimization process, SERS substrates formed by silver nanoparticles dispersed in paper and colloidal suspension will be evaluated, as well as portable and benchtop Raman equipment, to compare the intensities and the reproducibility of the SERS signal. We emphasize that the reproducibility of the SERS signal for analytical applications (SERS signal intensity vs target molecule concentration) is a challenge for the area, since such intensity depends on the homogeneity of the SERS substrate, mainly in terms of the shape and spatial distribution of the metallic nanoparticles. This project is linked to the Thematic Project FAPESP 2018/22214-6 "Towards the convergence of technologies: from sensors and biosensors to information visualization and machine learning for data analysis in clinical diagnostics" and intends to perform a BEPE internship at the Universidad Austral de Chile under the supervision of the Professor Dr. Igor Orlando Osório Román, an environmental chemist specializing in plasmonics applied to spectroscopy.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA, TATIANA A.; MARTIN, CIBELY S.; RUBIRA, RAFAEL J. G.; DE BARROS, ANERISE; MAZALI, ITALO O.; CONSTANTINO, CARLOS J. L.. Influence of L-Dopa adsorption time and concentration on Au nanorods aggregation for SERS quantitative analysis. CURRENT APPLIED PHYSICS, v. 54, p. 7-pg., . (22/04020-5, 17/15019-0, 20/05423-0, 18/22214-6)

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