Lipid membrane structure by SAXS: scattering density profiles from chemical groups

• Grant number: 22/07024-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2022
• End date: November 01, 2024
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Rosangela Itri
• Grantee: Samuel Utrabo Pizzol
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 23/15786-1 - Lipid membrane structure by small-angle X-ray scattering (SAXS):scattering density profiles of asymmetric leaflet chemical groups, BE.EP.IC

Abstract

The Scatter Density Profile (MSDP) Method will be used to simulate and analyze SAXS curves of symmetrical and asymmetrical model membranes (lipid bilayers), composed of different lipids including POPC, DOPC, sphingomyelin, cholesterol, POPE, POPS, simulating plasma membranes, and BMP simulating lysosomal membranes. For this, a database of chemical groups constituting the different lipids will be integrated into a ´program for analyzing flat bilayer and large unilamellar vesicles (LUVs) SAXS curves.(AU)