Analysis of the expression of BMP2, LTBP4 and DERL1 as a differential diagnosis between bending and malignant breast neoplasms of bitches in cytological samples

• Grant number: 22/01149-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2022
• End date: February 29, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Renee Laufer Amorim
• Grantee: Magna Gomes de Matos
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Mammary neoplasms are more common in non-neutered bitches and, in these, represent about 50 to 70% of all neoplasms. Diagnosis is often late, sometimes with aggressive behavior and metastases, in addition to short survival. Given the need to classify benign and malignant neoplasms, Klopfleisch et al. (2010) confirmed through laser microdissected frozen samples that the combined expression pattern of BMP2, LTBP4 and DERL1 is able to adequately stratify such tumors. Their results showed that BMP2 and DERL1 were overexpressed, while LTBP4 was underexpressed in those classified as malignant. Thus, it is justified to research the applicability of routine techniques in samples of canine breast tumors, verifying the ability to quantify gene expression, possibility of classification, serving as a substrate for comparative oncology research and development of by-products. The objective of this study is to standardize and determine the expression levels of the BMP2, DERL1 and LTBP4 genes of cytological samples of breast neoplasms, collected in the routine diagnosis of breast tumors of the pathology service, FMVZ, Unesp, Botucatu by qRT-PCR to differentiate them between benign and evil. Then, the expression levels obtained will be compared with the levels of normal breast tissue, benign neoplastic, and with the results of cytological and histopathological examination (gold standard diagnosis). This is a first step towards developing a product to be used as a diagnostic aid, which can accelerate the diagnostic process in clinical practice. (AU)