Metalloproteomic study of selenium in Psectrogaster amazonica and Raphiodon vulpinus from the Jirau region, Madeira River/Rondônia.

• Grant number: 22/04961-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2022
• End date: September 30, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Pedro de Magalhães Padilha
• Grantee: Beatriz Miotto
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The present proposal aims to identify protein and/or enzymatic selenium biomarkers in the species Psectrogaster amazonica (branquinha, non-carnivorous species) and Raphiodon vulpinus (curvina, carnivorous species) from the Jirau region, Madeira River/Rondônia. To achieve the proposed objectives, metalloproteomic strategies (2D PAGE - GFAAS - LC-MS/MS), Shotgun - LC-MS/MS and Bioinformatics will be used to: - Fractionate the proteome from muscle and liver tissue samples of the species Psectrogaster amazonica and Raphiodon vulpinus; Selenium map in protein spots of 2D PAGE gels; To characterize the selenium-associated protein spots in the muscle and liver proteome; Identify proteins associated with selenium and differentially expressed by Shotgun - LC-MS/MS. Based on the results of characterization of metal binding proteins and expression analysis of these, it is expected to elect possible selenium biomarkers in the species Psectrogaster amazonica and Raphiodon vulpinus from the Amazon region.