BIOIMPLANT OF BACTERIAL CELLULOSE MEMBRANE EXPERIMENTALLY IMPLANTED IN THE PERITONEUM OF WISTAR RATS - IN VIVO STUDY OF COLLAGEN FIBERS

• Grant number: 22/12590-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2023
• End date: December 31, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Alessandre Hataka
• Grantee: Rebecca Bertolo
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Traumatic injuries involving fraying and tissue loss in domestic animals are frequent and in several situations the healing, recovery and reconstruction of the tissues are not possible due to their extension, being necessary the use of bioimplants. The Bacterial Cellulose (BC) has been used for various applications in human medicine. However, in veterinary medicine, studies that investigate the morphological and immunohistochemical characteristics of the inflammatory and healing component in implanted BC in in vivo peritoneum were not observed. Still nowadays there is controversy over whether or not BC is biocompatible regarding the inflammatory and healing response. The objective of this work will be to evaluate the inflammatory and healing reaction caused by the BC membrane implanted intraperitoneally in Wistar rats using the technique of histopathology and immunohistochemistry of the inflammatory and healing component at the implant site. For that, 30 male or female Wistar rats, will be surgically implanted with BC in the peritoneal cavity, 15 animals will receive the BC and 15 will receive the sham group. After 60 days of postoperative, implant sites will be collected for histopathological and immunohistochemical analysis. The inflammatory and healing responses around BC will be evaluated, as well as the intensity and type of inflammatory infiltrate through histopathology and through immunohistochemical the involved types of collagen fibers and myofibroblasts in the process will be characterized. The obtained results will be spreadsheets and statistically analyzed to verify whether or not there are significant differences.