Tissue reaction after subcutaneous implantation of new biomaterial composed by bacterial cellulose produced by Gluconacetobacer xylinus

• Grant number: 13/21557-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): January 01, 2014
• Effective date (End): December 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Angela Mitie Otta Kinoshita
• Grantee: Gilberto Oréfice Marinho
• Host Institution: Centro de Ciências da Saúde. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil

Abstract

The reconstruction of bone tissue lost as a consequence of traumatic or pathological processes is a common problem in dentistry. Several techniques have been developed and improved, including the Guided Bone Regeneration (GBR) in which an occlusive membrane is used to seal the space of the bone defect, thus preventing the invasion of epithelial cells and connective tissue. A biomaterial that has been studied for use as occlusive membranes is bacterial cellulose (BC). The BC is produced from Gluconacetobacer xylinus and differs from plant cellulose, mainly due to its nanoscale fibers character, in contrast to vegetable micrometer character. BCs are extracted through the cell wall of G. xylinus, with a macroscopic structure that is mechanically and physically resistant, important feature of occlusive membrane. One of the first steps to test new materials for clinical use are tests of biocompatibility. Among these, the tissue response to subcutaneous implantation provides important information about the general response of the organism through the inflammatory pattern. Thus, the study of tissue response of this new biomaterial is necessary and valuable for future applications such as membrane occlusive procedure for GBR. Eighteen male rats (Rattus norvegicus Wistar) will be used, in which two incisions will be surgically made for subcutaneous implantation in the back of the material to be tested, CB and PTFE (Polytetrafluoroethylene), a commercial biocompatible material. After 7, 15 and 60 days post-surgery, 6 animals will be euthanized and tissue reaction microscopically analyzed observing the presence of necrosis, fibrous capsule and inflammatory cells. Six images will be recorded for each slide and the number of mononuclear, poliformonucleares and giant cells will be counted, as well as the thickness of the fibrous capsule. Quantitative data will be evaluated in accordance with a predetermined score and comparison of results will be conducted by the nonparametric Kruskal-Wallis and will be considered statistically different when p < 0:05. (AU)