Satellite DNAs, and the karyotype and ZZ/ZW sex chromosome differentiation in birds. Part IV. The harpy eagle (Harpia harpyja) as a model.

• Grant number: 22/14584-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2023
• End date: December 31, 2025
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Marcelo de Bello Cioffi
• Grantee: Guilherme Mota Souza
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 25/04254-4 - Comparative cytogenomics in birds: Extensive genomic reorganization uncovered by in silico analysis., BE.EP.IC 24/03030-2 - The impact of Chromosomal Reshuffling in the karyotype evolution of the red-legged seriema (Cariama cristata), BE.EP.IC

Abstract

This project aims to conduct a Satellitome investigation in the harpy eagle (Harpia harpyja), using both in situ and in silico mapping analyses in this species and other related ones. Recently, the harpy eagle genome was assembled at the chromosome level (NCBI GCF_026419915.1), indicating the presence of 58 linkage groups, which correspond to the 2n=58 described for the species. Therefore, it is a bird species with an extremely reduced 2n in relation to the ancestral karyotype (2n=80) due to extensive chromosomal fusion events that occurred. The entire collection of satellite DNAs (satellitome) from females will be isolated and characterized. Next, the study of the chromosomal distribution pattern of these satDNAs in males and females will allow the investigation of the chromosomal differentiation processes that occur during the chromosomal reshuffling that occurred in this species, as well as in the differentiation stages of the W chromosome. The results achieved will allow an improved understanding of the evolutionary processes that configured the distribution patterns of the repetitive sequences, as well as their contribution to the events of chromosomal rearrangements and evolution of the sex chromosomes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, GUILHERME MOTA; KRETSCHMER, RAFAEL; TOMA, GUSTAVO AKIRA; DE OLIVEIRA, ALAN MOURA; DEON, GEIZE APARECIDA; SETTI, PRINCIA GREJO; DOS SANTOS, RODRIGO ZENI; GOES, CAIO AUGUSTO GOMES; GARNERO, ANALIA DEL VALLE; GUNSKI, RICARDO JOSE; et al. Satellitome analysis on the pale-breasted thrush Turdus leucomelas (Passeriformes; Turdidae) uncovers the putative co-evolution of sex chromosomes and satellite DNAs. SCIENTIFIC REPORTS, v. 14, n. 1, p. 14-pg., 2024-09-04. (23/00955-2, 22/14584-3, 22/00427-3)

SOUZA, GUILHERME MOTA; VIDAL, JHON ALEX DZIECHCIARZ; UTSUNOMIA, RICARDO; DEON, GEIZE APARECIDA; DE OLIVEIRA, EDIVALDO HERCULANO CORREA; FRANCA, RAQUELI TERESINHA; PORTO-FORESTI, FABIO; LIEHR, THOMAS; DE SOUZA, FERNANDO HENRIQUE SANTOS; KRETSCHMER, RAFAEL; et al. Cytogenomic analysis in Seriemas (Cariamidae): Insights into an atypical avian karyotype. JOURNAL OF HEREDITY, v. 116, n. 4, p. 12-pg., 2025-05-12. (23/06898-0, 23/00955-2, 24/03030-2, 22/14584-3)