Treatment of critical bone defect in rat calvaria using particulate bone substitute associated or not with MTA.

• Grant number: 22/13118-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2023
• End date: February 28, 2025
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Idelmo Rangel Garcia Junior
• Grantee: Sara Alves Berton
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The use of homogeneous, xenogeneic and alloplastic biomaterials aims to restore volume and heightbone lost by physiological resorption after tooth loss. On the other hand, the MTA isa biocompatible material, composed of mineral oxides in the form of particleshydrophilic compounds used mainly as an endodontic cement that has propertiesantimicrobial properties and induces the formation of mineralized tissues. However, there are still shortcomingsof prospective studies of its use as a bone substitute or in association withthe other biomaterials. The objective of this study will be to evaluate the influence of the presence ofMTA Angelus Branco® on tissue response and mineralization and repair capacitytissue when associated with a xenogeneic bone substitute, in a critical defect in the calvariaof rats. For this, 70 Wistar males will be divided into 7 GCOA groups (GroupClot), GBIO (BIOSS Group), GOST (Osteosynt Group), GMTA (100% MTA Group),GMTA5 (Group MTA 5%), GMTA10 (Group MTA 10%), GMTA15 (Group MTA 15%) andevaluated in 2 times of euthanasia, 7 and 28 days through the analysis and quantification of theinflammatory response and histomorphometrics in HE staining. In addition, evaluatingusing the immunohistochemistry technique the following markers OCN, OPG, RANK-L; Evaluate andto quantify new bone formation in critical defects in rat calvaria; three specimensof each group at each time will be randomly selected to evaluate throughof the Micro-CT technique to bone neoformation in critical defects in the calvaria. The datawill be tabulated and submitted to the most appropriate statistical analysis, considering the levelsignificance of 5%.