In vitro protein synthesis tests for protein synthesis inhibitors for Trypanosoma cruzi

• Grant number: 22/11565-8
• Support Opportunities: Scholarships in Brazil - Program to Stimulate Scientific Vocations
• Start date: January 04, 2023
• End date: February 23, 2023
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Sergio Schenkman
• Grantee: Márcio Luan Ferreira Barros
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The main objective of this project is to test specific inhibitors of protein synthesis in vitro using Trypanosoma cruzi extracts, which can be used for the development of future treatment for Chagas disease. Assays using synthetic messenger RNA and detection of luciferase synthesis by assays have already been standardized in the laboratory. During the scholarship period, the student will be familiarized with the technique and test compounds from the Parasite Box that are part of a DNDi initiative to search for compounds for the treatment of CD. (AU)