Pathophysiological mechanisms responsible for Sepsis aggravation

• Grant number: 22/11909-9
• Support Opportunities: Scholarships in Brazil - Program to Stimulate Scientific Vocations
• Effective date (Start): January 09, 2023
• Effective date (End): February 13, 2023
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Fernando de Queiroz Cunha
• Grantee: Noa Dutkevicz
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The initial proposal of the Aristides Pacheco Leão Program for Stimulating Scientific Vocations is to allow undergraduate students to work in a research institution, under the guidance of a Brazilian Academy of Sciences member, promoting scientific development and stimulating scientific interest in undergraduates. Therefore, with this project we aim to enable the student to induce and identify sepsis induced by Cecal Ligation and Puncture (CLP) with different levels in BCl6/C57 strain mice. Sepsis is a hyper-inflammatory response characterized by the release of several pro-inflammatory mediators. It is the result of a complex process in which its mechanisms of action act on the regulatory pathways of the inflammatory, immune, hemostatic and neuroendocrine systems. Successful treatment of sepsis requires a better understanding of its pathophysiology and pathogenic mechanisms, which are the interaction with the immune system, pro-inflammatory and anti-inflammatory mechanisms, coagulation and anticoagulation, and changes in the patient's neuroendocrine response, so that it is possible to introduce appropriate prophylactic measures and therapeutic approaches. The student will be trained in the induction of acute sepsis by performing the CLP protocol, followed by the determination of animal survival, bacteremia, systemic cytokines and organ dysfunction, in particular the lungs, kidneys, liver and heart. Additionally, cardiac and vascular parameters will be monitored by telemetry to assess cardiac dysfunction during sepsis with the use of vasoconstrictors. (AU)