Scholarship 22/12483-5 - Citrus sinensis, Resistência genética - BV FAPESP
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CRISPR/Cas9 system in Sieve Element Occlusion c (SEOc) gene editing, aiming at obtaining citrus plants tolerant to Huanglongbing (HLB)

Grant number: 22/12483-5
Support Opportunities:Scholarships in Brazil - Master
Start date until: April 01, 2023
End date until: January 31, 2025
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Alessandra Alves de Souza
Grantee:Lucas Nascimento dos Santos
Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
Associated research grant:20/07045-3 - Biotechnological and genomic strategies for quality, productivity and sustainable management of citrus, coffee and sugar cane in the State of São Paulo, AP.NPOP
Associated scholarship(s):23/10399-0 - Enhancing sweet orange transformation through embryogenic cell cultures using CRISPR-Cas9 mediated editing of sieve element occlusion gene, BE.EP.MS

Abstract

Citrus species are among the main fruit trees in the world, it is one of the great crops that drive agribusiness. However, the productivity of orchards is constantly affected by pests and diseases that cause damage to the citrus industry. One of the most destructive diseases in orchards is Huanglongbing (HLB), caused by the gram-negative bacterium Candidatus Liberibacter asiaticus (CLas), which multiplies in phloem sieve vessels. From citrus-CLas interaction studies, genes associated with susceptibility to HLB, such as the Sieve Element Occlusion c (SEOc) gene, were identified. In addition to transcriptome data, reports indicating that the SEOc gene may be related to the development of symptoms in HLB-susceptible plants were also observed in the literature. In this perspective, SEOc becomes a target gene for genome editing, mediated by CRISPR/Cas9, however, it is necessary to develop efficient editing strategies for the success of the technique in citrus. In this sense, the editing/transformation of embryogenic cells has been shown to be an efficient method, since few cells with high rates of cell division will form somatic embryos, reducing the development of chimera plants. Thus, the purpose of this project is to improve the transformation/editing technique via CRISPR/Cas9, using embryogenic cells from citrus varieties to perform the SEOc gene editing aiming at HLB tolerance.

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