Genomic surveillance and viral quasispecies reconstruction of dengue virus in an endemic region

• Grant number: 22/16349-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2023
• End date: August 31, 2025
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Simone Kashima Haddad
• Grantee: Debora Glenda Lima de La-Roque
• Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil
• Associated research grant: 21/11944-6 - Continuous improvement of vaccines: Center for Viral Surveillance and Serological Assessment (CeVIVAS), AP.CCD
• Associated scholarship(s): 23/14976-1 - Analysis of viral diversity of Dengue virus 1 and 2 following antibody neutralization by plasma from previously infected individuals, BE.EP.DR

Abstract

Dengue is an arboviral fever caused by four distinct Dengue virus (DENV) serotypes, transmitted by the bite of Aedes mosquitoes. About 390 million infections occur annually in tropical and subtropical countries. Brazil has the highest number of DENV infections among Latin American countries. The northwest of the State of São Paulo is considered an endemic area for the infection. In this region, serotypes DENV1 and DENV2 are present. About 25% of DENV infections evolve into clinical manifestations, which can be classified as classic dengue or severe dengue. The main cause for the development of severe dengue is a secondary infection with a different DENV serotype. In RNA viruses such as DENV, viral subpopulations composed of similar but not identical genomes coexist in the same organism, called quasispecies. Studies have attempted to associate the diversity of quasispecies with clinical manifestations in primary or secondary dengue infections. We believe that the study of quasispecies of dengue in an endemic region may help understand the pathogenesis mechanisms of DENV infection, as well as in monitoring new viral variants.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LA-ROQUE, DEBORA GLENDA LIMA; SANTOS, ELAINE VIEIRA; OLIVEIRA, RENATA APARECIDA MACHADO; SLAVOV, SVETOSLAV NANEV; RODRIGUES, EVANDRA S.; FONSECA, VAGNER; MARTINS, ANTONIO JORGE; GIOMO, DENISE BERGAMASCHI; TORRES, PAULA MARILIA AFONSO; GENTIL, DANIELLE CRISTINA DACANAL; et al. DENV-1 genotype V circulation during the nonepidemic period in the Northeast of Sao Paulo State endemic area. Journal of Medical Virology, v. 96, n. 3, p. 4-pg., 2024-03-01. (13/08135-2, 21/11944-6, 22/16349-1)

POLICASTRO, LUCCA ROCHA; LIMA DE LA-ROQUE, DEBORA GLENDA; CALADO, RODRIGO TOCANTINS; COVAS, DIMAS TADEU; ALCANTARA, LUIZ CARLOS; GIOVANETTI, MARTA; KASHIMA, SIMONE. Oropouche virus in Brazil: Assessing the risks and challenges for transfusion medicine. Vox Sanguinis, v. N/A, p. 4-pg., 2024-12-24. (13/08135-2, 21/11944-6, 22/16349-1)

DE LA ROQUE, DEBORA GLENDA LIMA; SANTOS, ELAINE VIEIRA; POLICASTRO, LUCCA ROCHA; EVARISTO, MARIANE; DA COSTA, PERICLES NATAN MENDES; EVARISTO, MARRIANE; YAMAMOTO, APARECIDA YULIE; GIOMO, DENISE BERGAMASCHI; TORRES, PAULA MARILIA AFONSO; GENTIL, DANIELLE CRISTINA DACANAL; et al. Exploring the Chikungunya virus landscape in a dengue-endemic Brazilian area. JOURNAL OF INFECTION AND PUBLIC HEALTH, v. 17, n. 7, p. 4-pg., 2024-05-30. (22/16349-1, 13/08135-2, 21/11944-6)