Histological and systemic evaluation of the prophylactic effect of alcohol-free red wine consumption on the development of induced apical periodontitis in rats

• Grant number: 22/08087-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2023
• End date: March 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Felipe Haddad Martim Pederro
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The consumption of wine is a common habit all over the world, being one of the main sources of polyphenols through the diet. The administration of red wine reduces inflammation in apical periodontitis, but the alcohol present in the drink decreases its beneficial effect. Non-alcoholic red wine may be a promising alternative. However, there are no studies that relate the effect of non-alcoholic wine with apical periodontitis. The aim of this study will be to analyze and compare the development of apical periodontitis in rats with a normal diet or a diet supplemented with red wine consumption. Therefore, the prophylactic effect of non-alcoholic red wine consumption on the development of periapical lesions will be evaluated. Thus, 24 Wistar albino rats, all receiving BP induction, will be allocated into 3 groups as follows: Control (C), Red Wine without Alcohol (VTSA), Red Wine with Alcohol (VT), totaling 8 rats per group. The administration of the solutions for each group will be 4.28 mL/kg via gavage once a day, a volume corresponding to the amount of 300 mL (two glasses) of VT. During the entire experimental period, the animals will be weighed. After the experimental period, the animals will be euthanized for removal of the mandibles to be submitted to histological analysis of the bone tissue at the PA site. In addition, blood samples will be used for systemic analysis of the hematological profile and for the quantification of IL-10, IL-1², TNF-±, IL-17A, osteopontin, osteocalcin and alkaline phosphatase by ELISA. Statistical analysis will be performed using the SigmaPlot 12.0" program with a significance level of 5%.