Systemic toxicity of F18 bioglass. Analysis in hematological, hepatic, renal and cerebral tissues in Wistar rats.

• Grant number: 22/15097-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2023
• End date: February 28, 2027
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Mariana Pagliusi Justo
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated scholarship(s): 24/13424-8 - Investigation of Systemic Toxicity and Biocompatibility of Biomaterials Incorporated in GelMA Hydrogel in Rats., BE.EP.DR

Abstract

Endodontic materials are in intimate contact with vital tissues and may elicit different local and remote tissue reactions. This study has as its first objective to investigate the systemic toxicity of Bioglass F18, which has shown a high potential for clinical use in different endodontic treatments. Ca(OH)2 and MTA will be used as a standard for comparison. Moreover, the second objective is to analyze the possible systemic repercussion that these 3 materials have depending on the amount of material used, a fact that has never been considered in previous studies of this modality. We will use 112 Wistar rats divided into 8 groups: Group 1 and 2: control rats that will receive empty tubes. Group 3 and 4: rats receiving tubes containing Ca(OH)2. Group 5 and 6: rats which will receive tubes containing MTA. Group 7 and 8: rats that will receive tubes containing F18. The animals in the odd-numbered groups will receive only 1 tube each, while the animals in the even-numbered groups will receive 4 tubes filled with the selected material. After 7 and 30 days (n=7/time), hematological, liver, kidney and brain tissue will be collected as well as the tubes along with subcutaneous tissue. Local analysis will be performed on the subcutaneous connective tissue in response to the materials and on the hepatic, renal and cerebral tissues by hematoxylin and eosin staining to verify the inflammatory infiltrate and possible morphological alterations, and by PicroSirius Red staining to verify collagen maturation. The systemic analysis will be performed by evaluating the hematological profile (white and red series) and plasma biochemical profile quantifying alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, urea, creatinine, and microalbumin levels. We will also be able to measure ionized calcium in blood and liver, kidney and brain tissues and quantify the degree of systemic oxidative stress by analyzing the total antioxidant capacity (TAC), total oxidant concentration (TOC), lipid peroxidation (TBARS) of plasma and also the tissue oxidative stress (liver, kidney and brain) by measuring TBARS, the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx). Statistics will be performed according to each type of analysis and a 5% significance level will be considered (p <0.05).