COMBINED ANTIMICROBIAL ACTION OF PROPOLIS AND CINNAMON EXTRACTS ON CLINICAL STRAINS OF Acinetobacter baumannii

• Grant number: 23/03768-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2023
• Effective date (End): January 31, 2025
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Karoline Moura Chagas Martins
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Acinetobacter baumannii is an opportunistic pathogen often related mechanical ventilation-associated pneumonia, usually caused byaspiration of saliva containing such bacteria. Natural products are potent antimicrobial therapy and in view of the appearance of microorganismsmultiresistant to conventional medicines, the investment in research in this area is promising.The aim of the study is to evaluate the activity antimicrobial and antibiofilm effect of hydroalcoholic extracts of green propolis from Baccharis dracunculifolia and cinnamon (Cinnamomum verum) isolated and combined against clinical multidrug-resistant strains of Acinetobacter baumannii. For this purpose, plant extracts will be produced in our laboratory and evaluated for soluble solids content. For antimicrobial evaluation, the microdilution test of the broth will be carried out in accordance with the Clinical Norms and Laboratory Standards Institute (CLSI), the Checkerboard analysis, to evaluate combined effect of extracts and the antibiofilm activity will be evaluated by assay MTT. Data will be analyzed by ANOVA and Tukey's test, or by tests of Kruskal-Wallis and Dunn, considering a significance level of 5%.