Temporal proteomic analysis of OROV infection reveals manipulation of wide range of host proteins

• Grant number: 23/03946-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2023
• End date: September 23, 2024
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Luis Lamberti Pinto da Silva
• Grantee: Juan Oswaldo Concha Casaverde
• Supervisor: Colin Crump
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Cambridge, England
• Associated to the scholarship: 20/11900-6 - Study of the mechanism of Orthobunyavirus intracelular transport and externalization, BP.DR

Abstract

The Oropouche virus (OROV) is the etiological agent of Oropouche fever, a debilitating febrile illness common in South America. Despite its importance in public health, little is known about the biology of the virus in the host cell, especially the relationship between viral and cellular host protein. Our preliminary results from mass spectrometry analysis of the cellular proteome during OROV infection revealed several host proteins that are up or downregulated in response to the virus in human fibroblasts, including RNA polymerases, innate immunity signalling proteins and extracellular matrix proteins. The work proposed aims to elucidate the mechanism by which OROV modulates host proteins that are potentially involved in virus replication and pathogenesis and will complement my PhD study funded by FAPESP (2020/11900-6). (AU)