To enhance the osteoblastic differentiation of mesenchymal stem cells (MSCs) derived from adipose tissue, photobiomodulation (FBM) emerges as a non-invasive therapy that has been related to the acceleration of the repair process. However, the mechanisms by which these effects occur have not been elucidated. We hypothesize that the medium conditioned by MSCs treated with PBM increases osteoblast differentiation. Thus, MSCs derived from rat's adipose tissue will be separately seeded in plates and inserts and cultured in expansion or osteogenic medium, and the cells in the insert will be submitted to PBM (660 nm; 0,14 J; 20 mW; 0,714 W/cm2; 5 J/cm2). Non-irradiated MSCs will be used as control. Cell proliferation and migration, ALP activity and extracellular mineralized matrix production will be evaluated. Data will be compared by appropriate statistical tests with a significance level of 5%. The results of this study will contribute to the understanding of the intracellular mechanisms triggered by FBM and involved with the osteoblastic differentiation of MSCs, optimizing bone repair.
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