Standardization of latex and Iopamiron® arterial radiographic marking for applied anatomical studies of limbs in dogs

• Grant number: 23/06618-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2023
• End date: June 30, 2024
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Fabrício Singaretti de Oliveira
• Grantee: Giovanna Bobadilla Morelli
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Vascular markings are very important in anatomical studies as they allow better visualization of arteries and veins, facilitate dissection and make the preservation of vessels better. Among the vascular markers, silicone, latex, vinylic, acrylic, polyester and epoxy resins are the most used. The objective of this research is to standardize the radiographic arterial marking with latex and Iopamiron® associated, in the thoracic and pelvic limbs of dogs, in those very important regions in veterinary orthopedics. Sixteen cadavers of dogs between 4 and 10 kg will be used, in which the common carotid artery will be dissected and 120 mL/kg of curing salt will be injected (200g/L of sodium chloride, 10 g/L of nitrite and 10g/L of sodium nitrate), 120 mL/kg of alcohol with 5% glycerin. After the application of alcohol and curing salt, a solution composed of red latex mixed with Iopamiron®, a radiographic marker whose active ingredient is iopamidol, will be injected, in different proportions, to perform a radiographic examination. Arterial radiopacity will be evaluated in the thoracic and pelvic limbs that allow good radiographic identification and the bone region of the limbs, up to where the arteries are identified with the radiopaque marker. A table will be prepared indicating the exact bone location as far as the marker can be seen, as well as whether or not the dilution used was suitable for radiographic visualization.